Chater B V
Br J Haematol. 1976 Apr;32(4):515-24. doi: 10.1111/j.1365-2141.1976.tb00954.x.
Platelets from dogs decomplemented with purified Cobra Venom Factor (CVF) lose the ability to aggregate in response to collagen stimulation. In vitro in man, dog and rabbit, and in vivo in the dog, CVF abolishes the collagen response of platelets, the effect being dose-related. Resuspension of CVF inactivated platelets in plasma containing complement resulted in a total return of sensitivity to collagen. Examination of CVF inactivated platelets with the electron microscope failed to show any marked difference from control platelets. Serotonin granules were still present and the platelets retained a discoid appearance. Incubation of platelets with antibodies to C1, C3 and C5 resulted in inhibition of the collagen response: this effect was dose-related. Light microscopy indicated that DVF does not affect the adhesion of platelets to collagen but appears to prevent subsequent aggregation. It is suggested that the complement system is involved in the induction of the platelet release reaction by collagen, and that inhibition by CVF and anticomplement antibodies is the result of a blocking of the release reaction.
用纯化的眼镜蛇毒因子（CVF）进行去补体处理的犬血小板，失去了对胶原刺激产生聚集反应的能力。在人、犬和兔的体外实验以及犬的体内实验中，CVF消除了血小板对胶原的反应，且该效应与剂量相关。将经CVF处理而失活的血小板重悬于含有补体的血浆中，其对胶原的敏感性完全恢复。用电子显微镜检查经CVF处理失活的血小板，未发现与对照血小板有任何明显差异。5-羟色胺颗粒仍然存在，且血小板保持盘状外观。用针对C1、C3和C5的抗体孵育血小板会导致胶原反应受到抑制：该效应与剂量相关。光学显微镜检查表明，CVF不影响血小板与胶原的黏附，但似乎会阻止随后的聚集。提示补体系统参与了胶原诱导的血小板释放反应，而CVF和抗补体抗体的抑制作用是释放反应受阻的结果。
