Kulandaivelu G, Hall D O
Biochim Biophys Acta. 1976 Apr 9;430(1):46-52. doi: 10.1016/0005-2728(76)90220-6.
The addition of alpha-benzyl-alpha-bromomalodinitrile to different controlled states (non-phosphorylating [2]. phosphorylating [3], ATP-inhibited [4] and uncoupled) of photosynthetic electron transport to ferricyanide or benzoquinone demonstrate a significant inhibition in isolated spinach chloroplasts. alpha-Benzyl-alpha-bromomalodinitrile pretreatement of isolated chloroplasts or addition of alpha-benzyl-alpha-bromomalodinitrile at the onset of illumination completely abolished the O2 evolving reaction. The level of the steady state fluorescence in intact chloroplasts showed a alpha-benzyl-alpha-bromomalodinitrile concentration-dependent increase. The gradual decrease in the reoxidation capacity of the reduced quencher, Q with increasing alpha-benzyl-alpha-bromomalodinitrile concentrations provides evidence for an additional inhibitory site for alpha-benzyl-alpha-bromomalodinitrile between the two photosystems.
将α-苄基-α-溴代丙二腈添加到光合电子传递至铁氰化物或苯醌的不同控制状态（非磷酸化[2]、磷酸化[3]、ATP抑制[4]和解偶联）中，在分离的菠菜叶绿体中显示出显著抑制作用。对分离的叶绿体进行α-苄基-α-溴代丙二腈预处理或在光照开始时添加α-苄基-α-溴代丙二腈完全消除了O₂释放反应。完整叶绿体中稳态荧光水平呈现出α-苄基-α-溴代丙二腈浓度依赖性增加。随着α-苄基-α-溴代丙二腈浓度增加，还原猝灭剂Q的再氧化能力逐渐降低，这为α-苄基-α-溴代丙二腈在两个光系统之间存在额外抑制位点提供了证据。
