Jennings R C, Forti G
Biochim Biophys Acta. 1975 Jul 8;396(1):63-71. doi: 10.1016/0005-2728(75)90189-9.
Under conditions in which the Photosystem II quencher is rapidly reduced upon illumination, either after a preillumination or following treatment with dithionite, the fluorescence-induction curve of intact spinich chloroplasts (class I type) displays a pronounced dip. This dip is probably identical with that observed after prolonged anaerobic incubation of whole algal cells ("I-D dip"). It is inhibited by 3(3,4-dichlorophenyl)-1,1-dimethylurea and occurs in the presence of dithionite, sufficient to reduce the plastoquinone pool. It is influenced by far red light, methylviologen, anaerobiosis and uncouplers in a manner consistent with the interpretation that it represents a photochemical quenching of fluorescence by an electron transport component situated between the Photosystem II quencher and plastoquinone. Glutaraldehyde inhibition may indicate that protein structural changes are involved.
在光照后光系统II猝灭剂迅速被还原的条件下，无论是在预照光后还是用连二亚硫酸盐处理后，完整菠菜叶绿体（I类）的荧光诱导曲线都会出现明显的下降。这种下降可能与整个藻类细胞长时间厌氧培养后观察到的下降（“I-D下降”）相同。它受到3-(3,4-二氯苯基)-1,1-二甲基脲的抑制，并且在足以还原质体醌库的连二亚硫酸盐存在的情况下出现。它受到远红光、甲基紫精、厌氧和解偶联剂的影响，其方式与以下解释一致，即它代表位于光系统II猝灭剂和质体醌之间的电子传递成分对荧光的光化学猝灭。戊二醛抑制可能表明涉及蛋白质结构变化。
