利用纳秒荧光法对牛α-乳白蛋白和溶菌酶进行的比较研究。
A comparative study on bovine alpha-lactalbumin and lysozyme by nanosecond fluorometry.
作者信息
Tang L H, Kubota Y, Steiner R F
出版信息
Biophys Chem. 1976 Mar;4(2):203-13. doi: 10.1016/0301-4622(76)85011-9.
PMID:1260101
Abstract
Analysis of the time decay of fluorescence anisotropy of 1-dimethylaminoaphthalene-5-sulfonyl (DNS) and fluorescamine derivatives of bovine alpha-lactalbumin and lysozyme reveals that no significant differences in mean rotational relaxation times are present. While fluorescamine molecules appear to orient randomly on these proteins, DNS is bound with a preferential orientation. Other fluorescence characteristics of the labels are also cited.
摘要
对牛α-乳白蛋白和溶菌酶的1-二甲氨基萘-5-磺酰基（DNS）及荧光胺衍生物的荧光各向异性时间衰减分析表明，平均旋转弛豫时间不存在显著差异。虽然荧光胺分子似乎在这些蛋白质上随机取向，但DNS以优先取向结合。还列举了标记物的其他荧光特性。
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