DeChatelet L R, Shirley P S, Johnston R B
Blood. 1976 Apr;47(4):545-54.
The addition of 0.1 mug/ml of phorbol myristate acetate (PMA) to a suspension of resting human neutrophils causes a marked stimulation of all aspects of cellular oxidative metabolism normally associated with phagocytosis. PMA induces a greatly increased rate of glucose oxidation via the hexose monophosphate shunt, increased production of superoxide anion and of hydrogen peroxide, increased cellular chemiluminescence, and increased iodination of protein material. The time course of hexose monophosphate shunt activation and of chemiluminescence are similar to those observed following phagocytosis of opsonized zymosan; the levels of activation achieved in all cases approximate those seen following phagocytosis. These phenomena are not simply reflections of altered cellular permeability, since PMA actually inhibits the uptake of radioactive 2-deoxyglucose and of uniformly labeled amino acids. The addition of PMA similarly inhibits the uptake of 14C-labeled bacteria, suggesting a competition between the effect of the chemical and the process of phagocytosis. These results suggest that PMA activates the cell in the same manner as does phagocytosis. This compound should provide a useful tool for elucidating the metabolic events underlying the phenomena of phagocytosis and bacterial killing by polymorphonuclear leukocytes.
向静息人中性粒细胞悬液中添加0.1μg/ml佛波酯（PMA）可显著刺激通常与吞噬作用相关的细胞氧化代谢的各个方面。PMA通过磷酸己糖旁路诱导葡萄糖氧化速率大幅增加，超氧阴离子和过氧化氢的产生增加，细胞化学发光增加，以及蛋白质材料的碘化增加。磷酸己糖旁路激活和化学发光的时间进程与吞噬调理酵母聚糖后观察到的相似；在所有情况下达到的激活水平接近吞噬后所见水平。这些现象并非仅仅反映细胞通透性的改变，因为PMA实际上抑制放射性2-脱氧葡萄糖和均匀标记氨基酸的摄取。添加PMA同样抑制14C标记细菌的摄取，表明该化学物质的作用与吞噬过程之间存在竞争。这些结果表明PMA与吞噬作用以相同方式激活细胞。该化合物应为阐明多形核白细胞吞噬作用和细菌杀伤现象背后的代谢事件提供有用工具。
