Yin Ping, Burns Christopher J, Osman Peter D J, Cornell Bruce A
Australian Membrane Biotechnology Research Institute, 126 Greville Street, Chatswood NSW 2067, Australia.
Biosens Bioelectron. 2003 Apr;18(4):389-97. doi: 10.1016/s0956-5663(02)00160-4.
Alamethicin, a small transmembrane peptide, inserts into a tethered bilayer membrane (tBLM) to form ion channels, which we have investigated using electrical impedance spectroscopy. The number of channels formed is dependent on the incubation time, concentration of the alamethicin and the application of DC voltage. The properties of the ion channels when formed in tethered bilayers are similar to those for such channels assembled into black lipid membranes (BLMs). Furthermore, amiloride and certain analogs can inhibit the channel pores, formed in the tBLMs. The potency and concentration of the inhibitors can be determined by measuring the change of impedance. Our work illustrates the possibility of using a synthetic tBLM for the study of small peptide voltage dependent ion channels. A potential application of such a device is as a screening tool in drug discovery processes.
阿拉米辛是一种小的跨膜肽，它插入到拴系双层膜（tBLM）中形成离子通道，我们已用电阻抗谱对其进行了研究。形成的通道数量取决于孵育时间、阿拉米辛的浓度以及直流电压的施加。在拴系双层膜中形成的离子通道的特性与组装到黑脂质膜（BLM）中的此类通道的特性相似。此外，氨氯吡咪及某些类似物可抑制在tBLM中形成的通道孔。抑制剂的效力和浓度可通过测量阻抗变化来确定。我们的工作说明了使用合成tBLM研究小肽电压依赖性离子通道的可能性。这种装置的一个潜在应用是作为药物发现过程中的筛选工具。
