Wexler H, Sindelar W F, Ketcham A S
Cancer. 1976 Apr;37(4):1701-6. doi: 10.1002/1097-0142(197604)37:4<1701::aid-cncr2820370414>3.0.co;2-r.
The effects of immune serum on the survival of hematogenously disseminated malignant cells were investigated in six murine tumor systems with varying immunogenicity. Blood containing circulating tumor cells demonstrable by bioassay was obtained from donor mice with disseminated tumor. The cellular fraction was isolated by centrifugation and resuspended in immune serum obtained from donor animals bearing the same tumor (Group 1), in immune serum from mice bearing a differing tumor (Group 2), or in normal nonimmune serum (Group 3). The mixed cellular and serum fractions were intravenously inoculated into normal recipient mice which were subsequently scored for the development of pulmonary tumors. Similar incidence (35-50%) of pulmonary tumor deposits were seen in Groups 2 and 3. A significant enhancement in the incidence of lung tumors was present in the Group 1 animals for all tumor systems studies. It was suggested that the observed enhancement resulted from factors present in isologous immune serum but absent from both normal serum and immune serum obtained from hosts bearing heterologous tumors.
在六个具有不同免疫原性的小鼠肿瘤系统中，研究了免疫血清对血行播散的恶性细胞存活的影响。通过生物测定法可检测到含有循环肿瘤细胞的血液取自患有播散性肿瘤的供体小鼠。细胞部分通过离心分离，并重悬于来自患有相同肿瘤的供体动物的免疫血清（第1组）、来自患有不同肿瘤的小鼠的免疫血清（第2组）或正常非免疫血清（第3组）中。将混合的细胞和血清部分静脉注射到正常受体小鼠中，随后对其肺部肿瘤的发生情况进行评分。在第2组和第3组中观察到相似的肺肿瘤沉积发生率（35 - 50%）。在所有研究的肿瘤系统中，第1组动物的肺肿瘤发生率均显著提高。有人认为，观察到的这种提高是由同源免疫血清中存在但正常血清和来自携带异源肿瘤宿主的免疫血清中不存在的因素导致的。
