Moon Chang Hoon, Do Jeong Wan, Cha Seung Ju, Yoon Won Joon, Kim Seong Bum, Ko Myoung Seok, Park Myoung Ae, Kim Jin Woo, Sohn Sang Kyu, Lee Jong Hwa, Park Jeong Woo
Department of Biological Sciences, University of Ulsan, Ulsan 680-749, Korea.
Dis Aquat Organ. 2003 Jan 22;53(1):11-3. doi: 10.3354/dao053011.
In Korea, mass mortality occurred among cultured shrimp with visible macroscopic white spots in 2000, and we confirmed the presence of white spot syndrome virus (WSSV) in the tissues of moribund shrimp by electron microscopy. In order to identify the characteristics of this Korean isolate of WSSV, we cloned and characterized its genomic DNA coding for VP24, VP26, and VP28. On the nucleotide level, VP24, VP26, and VP28 of the Korean isolate were found to be 100%, 100%, and 99% identical to those of Taiwan, Thailand and Chinese isolates, respectively. On the deduced amino-acid level, all 3 virion proteins showed 100% identity to those of the foreign isolates. The extent of sequence identity suggests that the Korean isolate originated from the same ancestor as the Taiwanese, Thai and Chinese isolates.
2000年，韩国养殖的对虾出现大规模死亡，濒死对虾组织中可见明显的宏观白色斑点，我们通过电子显微镜确认了白斑综合征病毒（WSSV）的存在。为了鉴定这种韩国WSSV分离株的特征，我们克隆并表征了其编码VP24、VP26和VP28的基因组DNA。在核苷酸水平上，韩国分离株的VP24、VP26和VP28分别与台湾、泰国和中国分离株的VP24、VP26和VP28有100%、100%和99%的同一性。在推导的氨基酸水平上，所有3种病毒粒子蛋白与国外分离株的蛋白具有100%的同一性。序列同一性程度表明，韩国分离株与台湾、泰国和中国分离株起源于同一祖先。
