Hung Shan-Ling, Chen Yu-Ling, Chen Yen-Ting
Institute of Oral Biology and Faculty of Dentistry, National Yang-Ming University, Taipei, Taiwan.
J Periodontal Res. 2003 Apr;38(2):130-4. doi: 10.1034/j.1600-0765.2003.01652.x.
The effects of safrole on the defensive functions of human neutrophils were examined. At the concentrations employed in this study, safrole did not significantly affect the viability of peripheral blood neutrophils as verified by their ability to exclude trypan blue dye. However, exposure of neutrophils to safrole inhibited their bactericidal activity against oral pathogens, including Actinobacillus actinomycetemcomitans and Streptococcus mutans, in a dose dependent manner. In addition, safrole inhibited the production of bactericidal superoxide anion by neutrophils as measured by cytochrome c reduction. In conclusion, the results demonstrated that safrole reduced the antibacterial activity and the superoxide anion production of neutrophils. Inhibition of the defensive functions of neutrophils may be one possible mechanism by which safrole compromises the oral health.
研究了黄樟素对人中性粒细胞防御功能的影响。在本研究采用的浓度下，通过中性粒细胞排斥台盼蓝染料的能力验证，黄樟素对外周血中性粒细胞的活力没有显著影响。然而，中性粒细胞暴露于黄樟素会以剂量依赖的方式抑制其对口腔病原体（包括伴放线放线杆菌和变形链球菌）的杀菌活性。此外，通过细胞色素c还原测定，黄樟素抑制中性粒细胞产生杀菌性超氧阴离子。总之，结果表明黄樟素降低了中性粒细胞的抗菌活性和超氧阴离子的产生。抑制中性粒细胞的防御功能可能是黄樟素损害口腔健康的一种可能机制。
