Hu Xinhua, Zhang Qiang, Sun Daxin, Duan Peiyuan, Wang Xinwen, Duan Zhiquan
Department of Vascular Surgery, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zhonghua Yi Xue Za Zhi. 2002 Nov 25;82(22):1546-9.
To investigate the gene expression of nuclear transcriptional factor-kappa B (NF-kappa B) p65 and its inhibiting factor I kappa B in autogenous vein graft.
The right common jugular vein was transplanted to infrarenal abdominal aorta by microsurgical technique among 80 Wistar rats so as to establish an autogenous vein graft model. Ten vein graft samples were harvested 6 hours, 24 hours, 3 days, 7 days, 2 weeks, 4 weeks, 6 weeks, and 8 weeks after surgery. NF kappa B p65 mRNA and I kappa B beta mRNA were measured by reverse transcription-PCR and in situ hybridization. Western blotting and immunohistochemistry were used to detect the protein expression of NF kappa B p65 and I kappa B.
The expressions of NF kappa B p65 mRNA and I kappa B beta mRNA 6 hours after the surgery were 16% +/- 4% and 31% +/- 9% respectively (P < 0.01 vs. control vein). The expression of NF kappa B p65 mRNA reached in peak during the period 3 days to 7 days after the surgery (37% +/- 12% and 34% +/- 10% respectively, P< 0.01 vs. other teams), however, the I kappa B beta mRNA expression reached its peak during 1 to 2 weeks after the surgery (53% +/- 17% and 49% +/- 10% respectively, P < 0.01 vs. other teams). The expressions of NF kappa B p65 mRNA and I kappa B beta mRNA recovered to their baseline values 6 weeks after surgery. The expression of p65 protein reached its peak 1 week after the surgery (32% +/- 13%) and then decreased gradually. The expressions of I kappa B alpha and I kappa B beta decreased to 1/3 to 1/2 of the normal vein 6 hours to 24 hours after the surgery and then increased to 5 times that of the control vein 2 weeks after surgery (35% +/- 11% and 44% +/- 13% respectively).
The NF-kappa B/I kappa B system is activated in autogenous vein graft. The NF kappa B may become a new target for the prevention and therapy of intimal hyperplasia and stenosis after vein graft.
研究核转录因子-κB（NF-κB）p65及其抑制因子IκB在自体静脉移植中的基因表达。
采用显微外科技术将80只Wistar大鼠的右侧颈总静脉移植至肾下腹主动脉，建立自体静脉移植模型。术后6小时、24小时、3天、7天、2周、4周、6周和8周分别取10个静脉移植样本。采用逆转录-聚合酶链反应（RT-PCR）和原位杂交法检测NF-κB p65 mRNA和IκBβ mRNA。采用蛋白质印迹法和免疫组织化学法检测NF-κB p65和IκB的蛋白表达。
术后6小时NF-κB p65 mRNA和IκBβ mRNA的表达分别为16%±4%和31%±9%（与对照静脉相比，P<0.01）。NF-κB p65 mRNA的表达在术后3天至7天达到峰值（分别为37%±12%和34%±10%，与其他组相比，P<0.01），而IκBβ mRNA的表达在术后1至2周达到峰值（分别为53%±17%和49%±10%，与其他组相比，P<0.01）。术后6周NF-κB p65 mRNA和IκBβ mRNA的表达恢复至基线值。p65蛋白的表达在术后1周达到峰值（32%±13%），然后逐渐下降。IκBα和IκBβ的表达在术后6小时至24小时降至正常静脉的1/3至1/2，然后在术后2周升至对照静脉的5倍（分别为35%±11%和44%±13%）。
自体静脉移植中NF-κB/IκB系统被激活。NF-κB可能成为预防和治疗静脉移植后内膜增生和狭窄的新靶点。
