[胆汁中纳米细菌的培养与鉴定]

[Culture and identification of nanobacteria in bile].

作者信息

Li Yongguo, Wen Yu, Yang Zhulin, Wei Hong, Liu Wei, Tan Ailing, Wu Xiaoying, Wang Qunwei, Huang Shengfu, Kajander E O, Ciftcioglu N

机构信息

Second Xiangya Hospital, Central-South University, Changsha 410011, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2002 Nov 25;82(22):1557-60.

PMID:12609067

Abstract

OBJECTIVE

To study the distribution and identification of nanobacteria in bile and to evaluate the identifying methods of nanobacteria.

METHODS

RPMI1640 culture or RPMI1640 culture with 10% heat-inactivated gamma-FBS was added into 75 samples of cystic bile from gallbladders resected in operation. Nanobacteria were identified by immunohistochemical staining, transmission electron microscopy (TEM), and calcific staining.

RESULTS

Nanobacteria were found in 45 bile samples with a positive rate of 61.3%. The positive rate of nanobacteria was 75.7% among 37 bile samples with white precipitate adhering to the tube, and was 47.4% among the samples with flocculent precipitate or without precipitate (P < 0.05). The immunohistochemically confirmed presence of nanobacteria was re-confirmed by TEM in all the positive samples. The positive rate, sensitivity, specificity, false positive rate and false negative rate of calcific staining were 38.7%, 58.7%, 93.1%, 6.9% and 41.3% respectively.

CONCLUSION

Immunohistochemistry with monoclonal antibody of nanobacteria associated with TEM is useful in identifying nanobacteria. Calcific staining is of great value to identification of nanobacteria. Precipitation of white floccules adhering to the tube is an important microbiological characteristic of nanobacteria.

摘要

目的

研究纳米细菌在胆汁中的分布与鉴定情况，并评估纳米细菌的鉴定方法。

方法

将RPMI1640培养液或添加10%热灭活γ -胎牛血清的RPMI1640培养液加入75份手术切除胆囊的胆囊胆汁样本中。通过免疫组织化学染色、透射电子显微镜（TEM）和钙化染色对纳米细菌进行鉴定。

结果

在45份胆汁样本中发现纳米细菌，阳性率为61.3%。在37份有白色沉淀附着于管壁的胆汁样本中纳米细菌阳性率为75.7%，在有絮状沉淀或无沉淀的样本中阳性率为47.4%（P < 0.05）。所有阳性样本经免疫组织化学证实存在的纳米细菌通过TEM再次得到证实。钙化染色的阳性率、敏感性、特异性、假阳性率和假阴性率分别为38.7%、58.7%、93.1%、6.9%和41.3%。