Wang Qi, An Lijia, Chen Yuhua, Yue Shichang
Department of Respiratory Medicine, the Second Hospital affiliated to Dalian Medical University, Dalian 116023, China.
Chin Med J (Engl). 2002 Nov;115(11):1615-9.
To investigate the relationship between the expression of glucose regulated protein 94 (GRP94) at the level of mRNA and protein in vivo and in human lung cancer.
RT-PCR, immunohistochemistry and/or Western blot were used in 54 cases of lung cancer tissues and corresponding normal lung tissues.
There was a significant overexpression of GRP94 mRNA and protein in lung cancer tissues as compared with lung normal tissues. In lung cancer tissue, the relative level of GRP94 mRNA as evaluated by RT-PCR was 3.48 +/- 2.06, the level of GRP94 protein as evaluated by immunohistochemistry was + + to + + +, and by Western blot was 256.7 +/- 80.6. In lung normal tissue, the relative level of GRP94 mRNA was 2.01 +/- 1.83, the level of GRP94 protein was + to + + and 108.1 +/- 42.3. The differences in expression of GRP94 between the two tissues were significant (P < 0.05). Furthermore, the over-expression of GRP94 in the lung cancer tissues was correlated to grade of differentiation and stage of tumors. There was stronger expression in poor-differentiated tumors than in mild-to-high differentiated tumors (P < 0.05). There was also a stronger expression in stage III than in stage I and II tumors (P < 0.05). No statistically significant difference was found among various pathological types of tumors.
GRP94 was related with the occurrence, differentiation and progress of human lung cancer. Ascertaining the levels of GRP94 mRNA and protein may be valuable in evaluating the grade of differentiation and clinical stage of human lung cancer.
探讨葡萄糖调节蛋白94（GRP94）在体内及人肺癌组织中mRNA和蛋白水平的表达关系。
对54例肺癌组织及相应正常肺组织采用逆转录聚合酶链反应（RT-PCR）、免疫组织化学和/或蛋白质印迹法。
与正常肺组织相比，肺癌组织中GRP94 mRNA和蛋白显著过表达。在肺癌组织中，RT-PCR评估的GRP94 mRNA相对水平为3.48±2.06，免疫组织化学评估的GRP94蛋白水平为++至+++，蛋白质印迹法评估的为256.7±80.6。在正常肺组织中，GRP94 mRNA相对水平为2.01±1.83，GRP94蛋白水平为+至++，为108.1±42.3。两种组织中GRP94表达差异有统计学意义（P < 0.05）。此外，肺癌组织中GRP94的过表达与肿瘤的分化程度和分期相关。低分化肿瘤中的表达强于中高分化肿瘤（P < 0.05）。III期肿瘤中的表达也强于I期和II期肿瘤（P < 0.05）。不同病理类型的肿瘤之间未发现统计学显著差异。
GRP94与人肺癌的发生、分化及进展相关。确定GRP94 mRNA和蛋白水平可能对评估人肺癌的分化程度和临床分期有价值。
