Slit和robo：在肺发育中的表达模式。

Slit and robo: expression patterns in lung development.

作者信息

Anselmo Mark A, Dalvin Sussie, Prodhan Parthak, Komatsuzaki Katsumi, Aidlen Jeremy T, Schnitzer Jay J, Wu Jane Y, Kinane T Bernard

机构信息

Pediatric Pulmonary Unit, Massachusetts General Hospital for Children, Harvard Medical School, Jackson 14, GRJ 1416, 55 Fruit St, Boston, MA 02114, USA.

出版信息

Gene Expr Patterns. 2003 Mar;3(1):13-9. doi: 10.1016/s1567-133x(02)00095-9.

DOI:10.1016/s1567-133x(02)00095-9

PMID:12609596

Abstract

First described as an axonal guidance cue through its repulsive effect on neurons expressing its receptor Roundabout (Robo), the Slit ligand has effects on cell migration, axon branching and elongation. Indirect evidence implicates Slit and Robo in lung development. We now demonstrate that Slit-2 and Slit-3 are developmentally regulated in embryonic murine lung. Immunohistochemistry demonstrates Slit-2 and Slit-3 expression by the pulmonary mesenchyme and airway epithelium. Robo-1 and Robo-2 are also expressed by the developing mesenchyme and airway epithelium. As lung development progresses, Robo-1 and Robo-2 expression localizes to only the airway epithelium. We conclude Slit/Robo are expressed in temporo-spatially adjacent domains suggesting interactive roles in pulmonary bronchiolar development.

摘要

Slit配体最初被描述为一种轴突导向信号，它对表达其受体Roundabout（Robo）的神经元具有排斥作用，对细胞迁移、轴突分支和伸长也有影响。间接证据表明Slit和Robo参与肺发育。我们现在证明，Slit-2和Slit-3在胚胎期小鼠肺中受到发育调控。免疫组织化学显示，肺间充质和气道上皮表达Slit-2和Slit-3。发育中的间充质和气道上皮也表达Robo-1和Robo-2。随着肺发育的进展，Robo-1和Robo-2的表达仅定位于气道上皮。我们得出结论，Slit/Robo在时空相邻区域表达，提示它们在肺细支气管发育中具有相互作用的角色。