Carr Lauren, Parkinson David B, Dun Xin-Peng
Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth, Devon, United Kingdom.
Hubei University of Science and Technology, Xian-Ning City, Hubei, China.
PLoS One. 2017 Feb 24;12(2):e0172736. doi: 10.1371/journal.pone.0172736. eCollection 2017.
The secreted glycoproteins, Slit1-3, are classic axon guidance molecules that act as repulsive cues through their well characterised receptors Robo1-2 to allow precise axon pathfinding and neuronal migration. The expression patterns of Slit1-3 and Robo1-2 have been most characterized in the rodent developing nervous system and the adult brain, but little is known about their expression patterns in the adult rodent peripheral nervous system. Here, we report a detailed expression analysis of Slit1-3 and Robo1-2 in the adult mouse sciatic nerve as well as their expression in the nerve cell bodies within the ventral spinal cord (motor neurons) and dorsal root ganglion (sensory neurons). Our results show that, in the adult mouse peripheral nervous system, Slit1-3 and Robo1-2 are expressed in the cell bodies and axons of both motor and sensory neurons. While Slit1 and Robo2 are only expressed in peripheral axons and their cell bodies, Slit2, Slit3 and Robo1 are also expressed in satellite cells of the dorsal root ganglion, Schwann cells and fibroblasts of peripheral nerves. In addition to these expression patterns, we also demonstrate the expression of Robo1 in blood vessels of the peripheral nerves. Our work gives important new data on the expression patterns of Slit and Robo family members within the peripheral nervous system that may relate both to nerve homeostasis and the reaction of the peripheral nerves to injury.
分泌型糖蛋白Slit1 - 3是经典的轴突导向分子，它们通过其特征明确的受体Robo1 - 2发挥排斥性信号作用，以实现精确的轴突路径寻找和神经元迁移。Slit1 - 3和Robo1 - 2的表达模式在啮齿动物发育中的神经系统和成年大脑中研究得最为充分，但它们在成年啮齿动物外周神经系统中的表达模式却知之甚少。在此，我们报告了Slit1 - 3和Robo1 - 2在成年小鼠坐骨神经中的详细表达分析，以及它们在腹侧脊髓（运动神经元）和背根神经节（感觉神经元）内神经细胞体中的表达情况。我们的结果表明，在成年小鼠外周神经系统中，Slit1 - 3和Robo1 - 2在运动神经元和感觉神经元的细胞体及轴突中均有表达。虽然Slit1和Robo2仅在外周轴突及其细胞体中表达，但Slit2、Slit3和Robo1也在背根神经节的卫星细胞、外周神经的施万细胞和成纤维细胞中表达。除了这些表达模式外，我们还证明了Robo1在外周神经血管中的表达。我们的工作为外周神经系统中Slit和Robo家族成员的表达模式提供了重要的新数据，这些数据可能与神经稳态以及外周神经对损伤的反应有关。
