用于舒他西林生产过程中控的高效液相色谱法。

HPLC for in-process control in the production of sultamicillin.

作者信息

Laviana L, Fernández-Marí F, Bayod M, Blanco D

机构信息

MIT Fellowship at Asturpharma S.A., Silvota, Llanera, Asturias, Spain.

出版信息

J Pharm Biomed Anal. 2003 Feb 26;31(2):321-8. doi: 10.1016/s0731-7085(02)00642-8.

DOI:10.1016/s0731-7085(02)00642-8

PMID:12609671

Abstract

A high-performance liquid chromatographic assay coupled with UV detection (215 nm) was developed for the determination of sultamicillin and its synthesis precursors. The separation of the analytes was performed on a Kromasil C(18) column (15 cm x 4.6 mm i.d., 5 microm) at 20 degrees C. The mobile phase (25 mM phosphate buffer, pH 7.0 and acetonitrile 48%) was pumped at a flow rate of 1.0 ml min(-1). This method is sensitive (limits of detection ranged between 0.4 and 1.2 mg l(-1)) and selective for the determination of sultamicillin and could be used for monitoring different synthetic routes.

摘要

建立了一种结合紫外检测（215nm）的高效液相色谱法，用于测定舒他西林及其合成前体。在20℃下，于Kromasil C(18)柱（15cm×4.6mm内径，5μm）上进行分析物的分离。流动相（25mM磷酸盐缓冲液，pH 7.0和48%乙腈）以1.0ml min(-1)的流速泵送。该方法灵敏（检测限在0.4至1.2mg l(-1)之间），对舒他西林的测定具有选择性，可用于监测不同的合成路线。