在没有EBV质粒的情况下，爱泼斯坦-巴尔病毒（EBV）核抗原1与细胞复制灶共定位。
Epstein-Barr virus (EBV) nuclear antigen 1 colocalizes with cellular replication foci in the absence of EBV plasmids.
作者信息
Ito Sayuri, Yanagi Kazuo
机构信息
Herpesvirus Laboratory, Department of Virology I, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, Japan.
出版信息
J Virol. 2003 Mar;77(6):3824-31. doi: 10.1128/jvi.77.6.3824-3831.2003.
Epstein-Barr virus (EBV) EBNA-1 is the only EBV-encoded protein that is essential for the once-per-cell-cycle replication and maintenance of EBV plasmids in latently infected cells. EBNA-1 binds to the oriP region of latent EBV plasmids and cellular metaphase chromosomes. In the absence of oriP-containing plasmids, EBNA-1 was highly colocalized with cellular DNA replication foci that were identified by immunostaining S-phase cells for proliferating cell nuclear antigen and replication protein A (RP-A) in combination with DNA short pulse-labeling. For the association of EBNA-1 with the cellular replication focus areas, the EBNA-1 regions of amino acids (aa) 8 to 94 and/or aa 315 to 410, but not the RP-A-interacting carboxy-terminal region, were necessary. These results suggest a new aspect of latent virus-cell interactions.
爱泼斯坦-巴尔病毒（EBV）的EBNA-1是唯一一种对潜伏感染细胞中EBV质粒的每细胞周期一次复制和维持必不可少的EBV编码蛋白。EBNA-1与潜伏EBV质粒的oriP区域和细胞中期染色体结合。在没有含oriP质粒的情况下，EBNA-1与细胞DNA复制焦点高度共定位，这些焦点是通过对S期细胞进行免疫染色以检测增殖细胞核抗原和复制蛋白A（RP-A）并结合DNA短脉冲标记来确定的。对于EBNA-1与细胞复制焦点区域的关联，氨基酸（aa）8至94和/或aa 315至410的EBNA-1区域是必需的，但与RP-A相互作用的羧基末端区域不是必需的。这些结果提示了潜伏病毒与细胞相互作用的一个新方面。
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