爱泼斯坦-巴尔病毒核抗原1的DNA分离机制
The DNA segregation mechanism of Epstein-Barr virus nuclear antigen 1.
作者信息
Wu H, Ceccarelli D F, Frappier L
机构信息
Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada.
出版信息
EMBO Rep. 2000 Aug;1(2):140-4. doi: 10.1093/embo-reports/kvd026.
Abstract
Latent Epstein-Barr virus (EBV) genomes are maintained in human cells as low copy number episomes that are thought to be partitioned by attachment to the cellular mitotic chromosomes through the viral EBNA1 protein. We have identified a human protein, EBP2, which interacts with the EBNA1 sequences that govern EBV partitioning. Here we show that, in mitosis, EBP2 localizes to the condensed cellular chromosomes producing a staining pattern that is indistinguishable from that of EBNA1. The localization of EBNA1 proteins with mutations in the EBP2 binding region was also examined. An EBNA1 mutant (delta325-376) disrupted for EBP2 binding and segregation function was nuclear but failed to attach to the cellular chromosomes in mitosis. Our results indicate that amino acids 325-376 mediate the binding of EBNA1 to mitotic chromosomes and strongly suggest that EBNA1 mediates EBV segregation by attaching to EBP2 on the cellular mitotic chromosomes.
摘要
潜伏的爱泼斯坦-巴尔病毒（EBV）基因组以低拷贝数附加体的形式存在于人类细胞中，人们认为这些附加体通过病毒EBNA1蛋白附着于细胞有丝分裂染色体上而得以分配。我们鉴定出一种人类蛋白EBP2，它与控制EBV分配的EBNA1序列相互作用。在此我们表明，在有丝分裂过程中，EBP2定位于浓缩的细胞染色体上，产生的染色模式与EBNA1的染色模式无法区分。我们还研究了EBP2结合区域发生突变的EBNA1蛋白的定位情况。一个因EBP2结合和分离功能受损的EBNA1突变体（delta325 - 376）位于细胞核中，但在有丝分裂过程中未能附着于细胞染色体上。我们的结果表明，氨基酸325 - 376介导EBNA1与有丝分裂染色体的结合，并强烈提示EBNA1通过附着于细胞有丝分裂染色体上的EBP2来介导EBV的分离。
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本文引用的文献
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The budding yeast homolog of the human EBNA1-binding protein 2 (Ebp2p) is an essential nucleolar protein required for pre-rRNA processing.人类EBNA1结合蛋白2（Ebp2p）在出芽酵母中的同源物是一种前体核糖体RNA加工所需的重要核仁蛋白。
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The minimal replicator of Epstein-Barr virus oriP.爱泼斯坦-巴尔病毒oriP的最小复制子。
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Epstein-barr virus nuclear antigen-1 binds to nuclear transporter karyopherin alpha1/NPI-1 in addition to karyopherin alpha2/Rch1.爱泼斯坦-巴尔病毒核抗原-1除了与核转运蛋白核转运受体α2/Rch1结合外，还与核转运蛋白核转运受体α1/NPI-1结合。
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EBP2, a human protein that interacts with sequences of the Epstein-Barr virus nuclear antigen 1 important for plasmid maintenance.EBP2，一种与对质粒维持至关重要的爱泼斯坦-巴尔病毒核抗原1序列相互作用的人类蛋白质。
J Virol. 1999 Apr;73(4):2587-95. doi: 10.1128/JVI.73.4.2587-2595.1999.
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An imperfect correlation between DNA replication activity of Epstein-Barr virus nuclear antigen 1 (EBNA1) and binding to the nuclear import receptor, Rch1/importin alpha.爱泼斯坦-巴尔病毒核抗原1（EBNA1）的DNA复制活性与和核输入受体Rch1/输入蛋白α的结合之间存在不完全相关性。
Virology. 1997 Dec 22;239(2):340-51. doi: 10.1006/viro.1997.8874.
7
Human RPA (hSSB) interacts with EBNA1, the latent origin binding protein of Epstein-Barr virus.人类RPA（hSSB）与EB病毒的潜伏性起始结合蛋白EBNA1相互作用。
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P32/TAP, a cellular protein that interacts with EBNA-1 of Epstein-Barr virus.P32/TAP，一种与爱泼斯坦-巴尔病毒的EBNA-1相互作用的细胞蛋白。
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9
Epstein-Barr virus nuclear antigen 1 forms a complex with the nuclear transporter karyopherin alpha2.爱泼斯坦-巴尔病毒核抗原1与核转运蛋白核转运受体α2形成复合物。
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