Ferguson E, Vaughan A, Swale J
Clin Chim Acta. 1976 Mar 15;67(3):281-6. doi: 10.1016/0009-8981(76)90336-3.
A simple titrimetric method for the determination of total calcium in serum or heparinized plasma is described. After removing interfering substances with an ion-exchange resin, calcium is reacted with glyoxalbis (2-hydroxyanil), GBA, and the resulting red colour titrated with ethylenediaminetetra-acetic acid in the presence of a methanol/glycerol mixture. No special equipment is used, deproteinization is not necessary and only 0.2 ml of sample is required.
本文描述了一种测定血清或肝素化血浆中总钙的简单滴定法。用离子交换树脂去除干扰物质后，钙与乙二醛双（2-羟基苯胺）（GBA）反应，在甲醇/甘油混合物存在下，用乙二胺四乙酸滴定产生的红色。该方法无需使用特殊设备，无需进行脱蛋白处理，仅需0.2 ml样品。
