Maleewong W, Intapan P M, Wongkham C, Wongsaroj T, Kowsuwan T, Pumidonming W, Pongsaskulchoti P, Kitikoon V
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Parasitology. 2003 Jan;126(Pt 1):63-7. doi: 10.1017/s0031182002002573.
A PCR procedure for the detection of Opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes was developed. This procedure was based on primers designed from a pOV-A6 specific probe sequence giving a 330 base-pair product. The detection was accomplished in host tissue homogenates to which a single cercaria or metacercaria was introduced. PCR can detect as little as a single cercaria artificially inoculated in a snail or a single metacercaria artificially inoculated in a fish sample. The method gave a 100% positivity rate for all infected snails or fishes. The method did not yield a 330 base-pair amplified product with other digenean fluke DNAs such as Haplorchis taichui, Centrocestus spp., Echinostoma malayanum, Fasciola gigantica, animal schistosomes, Paragonimus heterotremus or Haplorchoides spp. The assay has great potential for application in epidemiological surveys of both snail and fish intermediate hosts as well as for investigation of foodborne parasites in freshwater fishes.
开发了一种用于检测实验感染的豆螺和鲤科鱼类中麝猫后睾吸虫的PCR方法。该方法基于从pOV-A6特异性探针序列设计的引物，可产生330个碱基对的产物。检测在引入单个尾蚴或囊蚴的宿主组织匀浆中进行。PCR能够检测到人工接种到蜗牛中的单个尾蚴或人工接种到鱼类样本中的单个囊蚴。该方法对所有感染的蜗牛或鱼类的阳性率均为100%。该方法不会用其他双腔吸虫DNA（如泰国棘口吸虫、棘口属、马来亚棘口吸虫、巨片形吸虫、动物血吸虫、异形吸虫或类后睾属）产生330个碱基对的扩增产物。该检测方法在蜗牛和鱼类中间宿主的流行病学调查以及淡水鱼食源性寄生虫调查中具有很大的应用潜力。
