微量SAGE具有高度代表性和可重复性，但揭示了从相似组织获取的样本间基因表达的主要差异。
MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues.
作者信息
Blackshaw Seth, Kuo Winston P, Park Peter J, Tsujikawa Motokazu, Gunnersen Jenny M, Scott Hamish S, Boon Wee-Ming, Tan Seong-Seng, Cepko Constance L
机构信息
Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute, 200 Longwood Avenue, Boston, MA 02115, USA.
出版信息
Genome Biol. 2003;4(3):R17. doi: 10.1186/gb-2003-4-3-r17. Epub 2003 Feb 18.
BACKGROUND
Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate.
RESULTS
We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals.
CONCLUSIONS
MicroSAGE is a reliable method of comprehensively profiling differences in gene expression among samples, but care should be taken in generalizing results obtained from libraries constructed from tissue obtained from different individuals and/or processed or stored differently.
背景
使用少量起始材料进行基因表达的序列分析（微量SAGE）尚未被确凿证明具有代表性、可重复性或准确性。
结果
我们表明微量SAGE具有高度代表性、可重复性和准确性，但从不同个体采集的组织样本之间在基因表达上存在明显差异。
结论
微量SAGE是全面分析样本间基因表达差异的可靠方法，但在推广从不同个体获得的组织构建的文库、或经过不同处理或储存的文库所得到的结果时应谨慎。
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