[胎盘细胞凋亡在胎儿生长受限中的作用]

[Role of placental apoptosis in fetal growth restriction].

作者信息

Liu Yuan, Gao Peng, Xie Yingbo, Wang Shuyun, Dai Minsheng, Jiang Sen

机构信息

Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2002 Dec;37(12):721-2.

PMID:12622913

Abstract

OBJECTIVE

To determine the relationship of placental cellular apoptosis and pathophysiology of fetal growth restriction (FGR).

METHODS

Placental samples were obtained from 18 pregnancies complicated by FGR and 14 normal pregnancies. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) and transmission electron microscopy were used to confirm the occurrence of apoptosis.

RESULTS

In FGR group the placental apoptosis rate was (n = 18) 12.1 per thousand, the average placental weight was (236 +/- 24) g, the average birth weight was (2,071 +/- 428) g; In normal group (n = 14), the placental apoptosis rate was 7.3 per thousand, the average placental weight was (354 +/- 63) g, the average birth weight was (3,411 +/- 588) g (P < 0.05). The incidence of apoptosis was significantly higher in placental samples from pregnancies with FGR compared with normal placental samples (P < 0.05). Under transmission election microscopy, apoptosis was obviously compact and the chromatins were formed as mass.

CONCLUSION

These results suggest that apoptosis may play a role in the pathophysiologic mechanisms of FGR.

摘要

目的

确定胎盘细胞凋亡与胎儿生长受限（FGR）病理生理学之间的关系。

方法

从18例合并FGR的妊娠和14例正常妊娠中获取胎盘样本。采用末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记法（TUNEL）和透射电子显微镜来确认凋亡的发生。

结果

FGR组（n = 18）胎盘凋亡率为千分之12.1，平均胎盘重量为（236 ± 24）g，平均出生体重为（2071 ± 428）g；正常组（n = 14）胎盘凋亡率为千分之7.3，平均胎盘重量为（354 ± 63）g，平均出生体重为（3411 ± 588）g（P < 0.05）。与正常胎盘样本相比，FGR妊娠胎盘样本中的凋亡发生率显著更高（P < 0.05）。在透射电子显微镜下，凋亡明显紧密，染色质形成团块。