Draghia-Akli Ruxandra, Khan Amir S, Cummings Kathleen K, Parghi Deena, Carpenter Robert H, Brown Patricia A
Advisys, Inc., 2700 Research Forest Drive, Suite 180, The Woodlands, Texas 77381, USA.
Technol Cancer Res Treat. 2002 Oct;1(5):365-72. doi: 10.1177/153303460200100507.
Electroporation has been shown to significantly increase plasmid transfer to the skeletal muscle, but this procedure is also implicated in muscle damage. We are reporting a highly efficient in vivo transfer of a plasmid formulated with poly-(L-glutamate) (PLG) into murine, canine and porcine muscle fibers using electric pulses of low field intensity. In mice and pigs, the use of secreted embryonic alkaline phosphatase (SEAP) as the indicator gene caused increased PLG expression by 2-3 fold compared to naked plasmid; while delivery of a PLG-plasmid formulation to dogs showed a 10-fold increase in serum SEAP levels compared to plasmid alone. Muscle lesions were reduced by the protective PLG. Thus, PLG may constitute a useful adjuvant for increased expression and reduced muscle trauma to plasmid DNA delivered by electroporation.
电穿孔已被证明能显著增加质粒向骨骼肌的转移，但该过程也与肌肉损伤有关。我们报告了一种高效的体内转染方法，使用低场强电脉冲将聚（L-谷氨酸）（PLG）配制的质粒导入小鼠、犬和猪的肌纤维。在小鼠和猪中，使用分泌型胚胎碱性磷酸酶（SEAP）作为指示基因，与裸质粒相比，PLG表达增加了2至3倍；而将PLG-质粒制剂递送至犬时，血清SEAP水平比单独使用质粒时增加了10倍。保护性的PLG减少了肌肉损伤。因此，PLG可能是一种有用的佐剂，可提高通过电穿孔递送的质粒DNA的表达，并减少肌肉创伤。
