输注的卵圆细胞对肝脏的选择性嗜性。
A selective tropism of transfused oval cells for liver.
作者信息
Chen Jian-Zhi, Hong Hai, Xiang Jin, Xue Ling, Zhao Guo-Qiang
机构信息
Department of Pathology, Medical School of Sun Yat-Sen University, 74 Zhongshan 2nd Rd., Guangzhou 510080, Guangdong Province, China.
出版信息
World J Gastroenterol. 2003 Mar;9(3):544-6. doi: 10.3748/wjg.v9.i3.544.
AIM
To explore the biological behaviors of hepatic oval cells after transfused into the circulation of experimental animals.
METHODS
Oval cells from male SD rat were transfused into the circulation of a female rat which were treated by a 2-AAF/CCl(4) program, through caudal vein. Sex-determining gene sry which located on Y chromosome was examined by PCR and in situ hybridization technique in liver, kidney and spleen of the experimental animals, respectively.
RESULTS
The results of the cell-transplant experiment showed that the sry gene was detectable only in the liver but not in spleen and kidney of the experimental rats, and no signals could be detected in the control animals. It can be also morphologically proved that some exogenous cells had migrated into the parenchyma of the liver and settled there.
CONCLUSION
The result means that there are exogenous cells located in the liver of the experimental animal and the localization is specific to the liver. This indicates that some "signal molecules" must exist in the circulation of the rats treated by 2-AAF/CCl(4). These "signal molecules" might play an important role in specific localization and differentiation of transfused oval cells.
目的
探讨肝卵圆细胞输注到实验动物循环系统后的生物学行为。
方法
将雄性SD大鼠的卵圆细胞经尾静脉输注到经2-乙酰氨基芴/四氯化碳处理的雌性大鼠的循环系统中。分别采用聚合酶链反应（PCR）和原位杂交技术检测实验动物肝脏、肾脏和脾脏中位于Y染色体上的性别决定基因sry。
结果
细胞移植实验结果显示，仅在实验大鼠的肝脏中可检测到sry基因，而在脾脏和肾脏中未检测到，且在对照动物中未检测到信号。从形态学上也可证明，一些外源细胞已迁移至肝脏实质并在那里定居。
结论
该结果表明实验动物肝脏中存在外源细胞，且这种定位具有肝脏特异性。这表明在经2-乙酰氨基芴/四氯化碳处理的大鼠循环系统中必定存在某些“信号分子”。这些“信号分子”可能在输注的卵圆细胞的特异性定位和分化中发挥重要作用。
Zotero 插件