Nakajima Kei, Yamashita Takeshi, Kusuhara Masatoshi, Yonemura Atsushi, Ito Toshimitsu, Higashi Kenji, Ayaori Makoto, Ohmori Reiko, Nakamura Haruo, Ohsuzu Fumio
First Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-0042, Japan.
Clin Biochem. 2003 Mar;36(2):113-20. doi: 10.1016/s0009-9120(02)00438-1.
Lipoprotein(a) [Lp(a)] can be oxidized by copper in vitro in a way comparable to low-density lipoprotein (LDL). We sought to determine whether the susceptibility of Lp(a) to oxidation is correlated with the susceptibility of autologous heterogeneous LDL, with apolipoprotein(a) [apo(a)] molecular size, or with both factors.
We examined shifts in electrophoretic mobility of Lp(a) and LDL caused by copper oxidation in plasma samples from 81 healthy men. The effect of copper oxidation on different-sized apo(a) was also evaluated.
There was a close correlation between the relative electrophoretic mobilities of oxidized Lp(a) and oxidized LDL in subjects, especially with small-sized apo(a) (n = 25, r = 0.72, p < 0.0001). Oxidative processes in Lp(a) resulted in the degradation of large-, but not small-sized apo(a).
The susceptibility of Lp(a) to oxidation is correlated with that of autologous LDL. Large-sized apo(a) may be involved in the Lp(a) oxidation.
脂蛋白(a)[Lp(a)]在体外可被铜氧化，其方式与低密度脂蛋白(LDL)类似。我们试图确定Lp(a)的氧化易感性是否与自体异质性LDL的氧化易感性、载脂蛋白(a)[apo(a)]分子大小或这两个因素相关。
我们检测了81名健康男性血浆样本中铜氧化引起的Lp(a)和LDL电泳迁移率的变化。还评估了铜氧化对不同大小apo(a)的影响。
受试者中氧化型Lp(a)和氧化型LDL的相对电泳迁移率之间存在密切相关性，尤其是与小尺寸apo(a)相关(n = 25，r = 0.72，p < 0.0001)。Lp(a)中的氧化过程导致大尺寸而非小尺寸apo(a)的降解。
Lp(a)的氧化易感性与自体LDL的氧化易感性相关。大尺寸apo(a)可能参与Lp(a)的氧化。
