Ma Hsu, Xu Ruian, Cheng Henrich, Kuo Huai-Sheng, During Matthew, Fang Rong-Hwang
School of Medicine, NationalYang-Ming University and Department of Neurosurgery, Veterans General Hospital-Taipei, Taiwan.
J Trauma. 2003 Mar;54(3):569-73. doi: 10.1097/01.TA.0000042016.45195.4C.
BACKGROUND Gene transfer is a new territory for clinicians. Intractable disorders might be approached in such a way. Adeno-associated virus (AAV) vector has been transfected successfully into a variety of tissues including skin. We evaluated the ability of this vector to transfer and cause expression of the reporter gene in human keloid tissue. METHODS Human keloid specimens were injected with an AAV vector encoding beta-galactosidase and incubated for 4 weeks after injection. The presence of mRNA and beta-galactosidase enzymatic activity were assayed by reverse-transcriptase polymerase chain reaction and the X-gal technique. RESULTS Gene expression shown by reverse-transcriptase polymerase chain reaction was observed in keloid tissue 4 weeks after injection, and so was the positive X-gal staining. CONCLUSION Our results showed that AAV vector could transduce human keloid tissue effectively. Replacement of the reporter gene with a functioning gene might be feasible for keloid treatment.
背景 基因转移对临床医生来说是一个新领域。可以用这种方法来治疗难治性疾病。腺相关病毒（AAV）载体已成功转染到包括皮肤在内的多种组织中。我们评估了这种载体在人瘢痕疙瘩组织中转移并导致报告基因表达的能力。
方法 向人瘢痕疙瘩标本中注射编码β-半乳糖苷酶的AAV载体，并在注射后孵育4周。通过逆转录聚合酶链反应和X-gal技术检测mRNA的存在和β-半乳糖苷酶的酶活性。
结果 注射后4周在瘢痕疙瘩组织中观察到逆转录聚合酶链反应显示的基因表达，X-gal染色也呈阳性。
结论 我们的结果表明，AAV载体可以有效地转导人瘢痕疙瘩组织。用功能基因替代报告基因可能对瘢痕疙瘩治疗可行。
