活细胞中胰岛素受体与蛋白酪氨酸磷酸酶1B之间相互作用的动力学
Dynamics of the interaction between the insulin receptor and protein tyrosine-phosphatase 1B in living cells.
作者信息
Boute Nicolas, Boubekeur Samira, Lacasa Danièle, Issad Tarik
机构信息
Department of Cell Biology, Institut Cochin, CNRS-UMR 8104, INSERM U567, Université Paris V, 22 Rue Méchain, 75014 Paris, France.
出版信息
EMBO Rep. 2003 Mar;4(3):313-9. doi: 10.1038/sj.embor.embor767.
Abstract
The dynamics of the interaction of the insulin receptor with a substrate-trapping mutant of protein-tyrosine phosphatase 1B (PTP1B) were monitored in living human embryonic kidney cells using bioluminescence resonance energy transfer (BRET). Insulin dose-dependently stimulates this interaction, which could be followed in real time for more than 30 minutes. The effect of insulin on the BRET signal could be detected at early time-points (30 seconds), suggesting that in intact cells the tyrosine-kinase activity of the insulin receptor is tightly controlled by PTP1B. Interestingly, the basal (insulin-independent) interaction of the insulin receptor with PTP1B was much weaker with a soluble form of the tyrosine-phosphatase than with the endoplasmic reticulum (ER)-targeted form. Inhibition of insulin-receptor processing using tunicamycin suggests that the basal interaction occurs during insulin-receptor biosynthesis in the ER. Therefore, localization of PTP1B in this compartment might be important for the regulation of insulin receptors during their biosynthesis.
摘要
利用生物发光共振能量转移（BRET）技术，在活的人胚肾细胞中监测了胰岛素受体与蛋白酪氨酸磷酸酶1B（PTP1B）底物捕获突变体之间的相互作用动力学。胰岛素呈剂量依赖性地刺激这种相互作用，这种相互作用可以实时跟踪30多分钟。在早期时间点（30秒）就能检测到胰岛素对BRET信号的影响，这表明在完整细胞中，胰岛素受体的酪氨酸激酶活性受PTP1B的严格控制。有趣的是，与内质网（ER）靶向形式相比，胰岛素受体与酪氨酸磷酸酶的可溶性形式之间的基础（不依赖胰岛素）相互作用要弱得多。用衣霉素抑制胰岛素受体加工表明基础相互作用发生在内质网中胰岛素受体生物合成过程中。因此，PTP1B在该区室中的定位可能对胰岛素受体生物合成过程中的调节很重要。
相似文献
1
Dynamics of the interaction between the insulin receptor and protein tyrosine-phosphatase 1B in living cells.活细胞中胰岛素受体与蛋白酪氨酸磷酸酶1B之间相互作用的动力学
EMBO Rep. 2003 Mar;4(3):313-9. doi: 10.1038/sj.embor.embor767.
2
Interaction of PTPB with the insulin receptor precursor during its biosynthesis in the endoplasmic reticulum.在内质网中，PTPB在胰岛素受体前体生物合成过程中与之发生相互作用。
Biochimie. 2005 Jan;87(1):111-6. doi: 10.1016/j.biochi.2004.12.008.
3
Protein tyrosine phosphatase-1B dephosphorylation of the insulin receptor occurs in a perinuclear endosome compartment in human embryonic kidney 293 cells.蛋白酪氨酸磷酸酶-1B对胰岛素受体的去磷酸化作用发生在人胚肾293细胞的核周内体区室中。
J Biol Chem. 2004 Mar 26;279(13):12868-75. doi: 10.1074/jbc.M309600200. Epub 2004 Jan 13.
4
Monitoring the activation state of the insulin-like growth factor-1 receptor and its interaction with protein tyrosine phosphatase 1B using bioluminescence resonance energy transfer.利用生物发光共振能量转移监测胰岛素样生长因子-1受体的激活状态及其与蛋白酪氨酸磷酸酶1B的相互作用。
Mol Pharmacol. 2005 Sep;68(3):885-94. doi: 10.1124/mol.105.013151. Epub 2005 Jun 23.
5
Protein tyrosine phosphatase 1B interacts with the activated insulin receptor.蛋白酪氨酸磷酸酶1B与活化的胰岛素受体相互作用。
Diabetes. 1996 Oct;45(10):1379-85. doi: 10.2337/diab.45.10.1379.
6
Interaction of the insulin receptor with the receptor-like protein tyrosine phosphatases PTPalpha and PTPepsilon in living cells.胰岛素受体与活细胞中类受体蛋白酪氨酸磷酸酶PTPα和PTPε的相互作用。
Mol Pharmacol. 2005 Apr;67(4):1206-13. doi: 10.1124/mol.104.009514. Epub 2005 Jan 3.
7
Interaction between the insulin receptor and Grb14: a dynamic study in living cells using BRET.胰岛素受体与Grb14之间的相互作用：利用生物发光共振能量转移技术在活细胞中的动态研究
Biochem Pharmacol. 2006 Nov 30;72(11):1355-66. doi: 10.1016/j.bcp.2006.07.018. Epub 2006 Aug 28.
8
Phosphorylation and activation of protein tyrosine phosphatase (PTP) 1B by insulin receptor.胰岛素受体对蛋白酪氨酸磷酸酶（PTP）1B的磷酸化及激活作用。
Mol Cell Biochem. 2001 May;221(1-2):147-54. doi: 10.1023/a:1010909031310.
9
A new highly efficient substrate-trapping mutant of protein tyrosine phosphatase 1B (PTP1B) reveals full autoactivation of the insulin receptor precursor.一种新型高效的蛋白酪氨酸磷酸酶 1B（PTP1B）底物捕获突变体揭示了胰岛素受体前体的完全自动激活。
J Biol Chem. 2011 Jun 3;286(22):19373-80. doi: 10.1074/jbc.M111.222984. Epub 2011 Apr 12.
10
Analysis of in vitro interactions of protein tyrosine phosphatase 1B with insulin receptors.蛋白酪氨酸磷酸酶1B与胰岛素受体的体外相互作用分析
Mol Cell Endocrinol. 2001 Feb 28;173(1-2):109-20. doi: 10.1016/s0303-7207(00)00402-0.
引用本文的文献
1
Increased O-GlcNAcylation promotes IGF-1 receptor/PhosphatidyI Inositol-3 kinase/Akt pathway in cervical cancer cells.糖基化修饰增加促进了宫颈癌细胞中的 IGF-1 受体/磷酯酰肌醇-3 激酶/蛋白激酶 B 通路。
Sci Rep. 2022 Mar 16;12(1):4464. doi: 10.1038/s41598-022-08445-0.
2
Role of stilbenes against insulin resistance: A review.芪类化合物对胰岛素抵抗的作用：综述
Food Sci Nutr. 2021 Sep 13;9(11):6389-6405. doi: 10.1002/fsn3.2553. eCollection 2021 Nov.
3
Junctional ER Organization Affects Mechanotransduction at Cadherin-Mediated Adhesions.连接区内质网的组织影响钙黏蛋白介导黏附处的机械转导。
Front Cell Dev Biol. 2021 Jun 17;9:669086. doi: 10.3389/fcell.2021.669086. eCollection 2021.
4
Identification of insulin-sensitizing molecules acting by disrupting the interaction between the Insulin Receptor and Grb14.鉴定通过破坏胰岛素受体和 Grb14 之间相互作用而起作用的胰岛素增敏分子。
Sci Rep. 2017 Dec 4;7(1):16901. doi: 10.1038/s41598-017-17122-6.
5
Phospho-Regulation of Soma-to-Axon Transcytosis of Neurotrophin Receptors.神经营养因子受体从胞体到轴突的转胞吞作用的磷酸化调节
Dev Cell. 2017 Sep 25;42(6):626-639.e5. doi: 10.1016/j.devcel.2017.08.009. Epub 2017 Sep 14.
6
Protein tyrosine phosphatase 1B expression contributes to the development of breast cancer.蛋白酪氨酸磷酸酶1B的表达促进乳腺癌的发展。
J Zhejiang Univ Sci B. 2017;18(4):334-342. doi: 10.1631/jzus.B1600184.
7
S-nitrosylation of endogenous protein tyrosine phosphatases in endothelial insulin signaling.内皮胰岛素信号传导中内源性蛋白质酪氨酸磷酸酶的S-亚硝基化作用
Free Radic Biol Med. 2016 Oct;99:199-213. doi: 10.1016/j.freeradbiomed.2016.08.012. Epub 2016 Aug 10.
8
UBC9-dependent association between calnexin and protein tyrosine phosphatase 1B (PTP1B) at the endoplasmic reticulum.在内质网中，钙连蛋白与蛋白酪氨酸磷酸酶1B（PTP1B）之间存在依赖泛素结合酶9（UBC9）的关联。
J Biol Chem. 2015 Feb 27;290(9):5725-38. doi: 10.1074/jbc.M114.635474. Epub 2015 Jan 13.
9
Demonstration of a direct interaction between β2-adrenergic receptor and insulin receptor by BRET and bioinformatics.通过生物发光能量共振转移（BRET）和生物信息学证明β2-肾上腺素能受体与胰岛素受体之间存在直接相互作用。
PLoS One. 2014 Nov 17;9(11):e112664. doi: 10.1371/journal.pone.0112664. eCollection 2014.
10
Protein-tyrosine phosphatase 1B substrates and metabolic regulation.蛋白酪氨酸磷酸酶1B底物与代谢调节。
Semin Cell Dev Biol. 2015 Jan;37:58-65. doi: 10.1016/j.semcdb.2014.09.020. Epub 2014 Sep 28.
本文引用的文献
1
Regulation of receptor tyrosine kinase signaling by protein tyrosine phosphatase-1B.蛋白酪氨酸磷酸酶-1B对受体酪氨酸激酶信号传导的调控
J Biol Chem. 2003 Jan 10;278(2):739-44. doi: 10.1074/jbc.M210194200. Epub 2002 Nov 6.
2
The use of resonance energy transfer in high-throughput screening: BRET versus FRET.共振能量转移在高通量筛选中的应用：生物发光共振能量转移与荧光共振能量转移的比较
Trends Pharmacol Sci. 2002 Aug;23(8):351-4. doi: 10.1016/s0165-6147(02)02062-x.
3
Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum.内质网表面上蛋白酪氨酸磷酸酶1B（PTP1B）使受体去磷酸化的成像位点。
Science. 2002 Mar 1;295(5560):1708-11. doi: 10.1126/science.1067566.
4
Rapid internalization and recycling of the human neuropeptide Y Y(1) receptor.人神经肽Y Y(1)受体的快速内化与再循环
J Biol Chem. 2002 Feb 22;277(8):6645-55. doi: 10.1074/jbc.M107224200. Epub 2001 Dec 7.
5
Monitoring the activation state of the insulin receptor using bioluminescence resonance energy transfer.利用生物发光共振能量转移监测胰岛素受体的激活状态。
Mol Pharmacol. 2001 Oct;60(4):640-5.
6
Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET).利用生物发光共振能量转移（BRET）检测活细胞中的β2-肾上腺素能受体二聚化。
Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3684-9. doi: 10.1073/pnas.97.7.3684.
7
Increased insulin sensitivity and obesity resistance in mice lacking the protein tyrosine phosphatase-1B gene.缺乏蛋白酪氨酸磷酸酶-1B基因的小鼠胰岛素敏感性增加及抗肥胖能力增强。
Science. 1999 Mar 5;283(5407):1544-8. doi: 10.1126/science.283.5407.1544.
8
Molecular basis of insulin action.胰岛素作用的分子基础。
Diabetes Metab. 1998 Dec;24(6):477-89.
9
A bioluminescence resonance energy transfer (BRET) system: application to interacting circadian clock proteins.一种生物发光共振能量转移（BRET）系统：应用于相互作用的生物钟蛋白。
Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):151-6. doi: 10.1073/pnas.96.1.151.
10
Growth factor activity of endothelin-1 in primary astrocytes mediated by adhesion-dependent and -independent pathways.内皮素-1在原代星形胶质细胞中的生长因子活性由黏附依赖性和非依赖性途径介导。
J Neurosci. 1997 Aug 15;17(16):6203-12. doi: 10.1523/JNEUROSCI.17-16-06203.1997.
Zotero 插件