Gupta Pawan, Khan Rizwan H, Saleemuddin M
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Uttar Pradesh, India.
Biochim Biophys Acta. 2003 Mar 21;1646(1-2):131-5. doi: 10.1016/s1570-9639(02)00554-x.
Antienzyme polyclonal antibodies against stem bromelain were raised in male albino rabbits and the Fab' monomers isolated from the IgG of the immune sera. Incubation of bromelain with the Fab' resulted in binding and gel filtration of the resulting complex suggested a 1:1 stoichiometry. Complexing with the Fab' resulted in significant stabilization of bromelain against thermal inactivation and alkaline pH.
针对茎菠萝蛋白酶的抗酶多克隆抗体在雄性白化兔中产生，并从免疫血清的IgG中分离出Fab'单体。菠萝蛋白酶与Fab'孵育导致结合，所得复合物的凝胶过滤表明化学计量比为1:1。与Fab'复合导致菠萝蛋白酶对热失活和碱性pH具有显著的稳定性。
