一种含有干扰素调节因子1A（IRF1A）启动子的鱼类特异性表达载体，用于鱼类的基因免疫。

A fish specific expression vector containing the interferon regulatory factor 1A (IRF1A) promoter for genetic immunization of fish.

作者信息

Alonso Marta, Johnson Marc, Simon Ben, Leong Jo-Ann

机构信息

Department of Microbiology, Center for Salmon Disease Research, Oregon State University, 220 Nash Hall, Corvallis, OR 97330, USA.

出版信息

Vaccine. 2003 Apr 2;21(15):1591-600. doi: 10.1016/s0264-410x(02)00749-1.

DOI:10.1016/s0264-410x(02)00749-1

PMID:12639480

Abstract

DNA plasmid vectors were constructed with inducible rainbow trout promoters to take the place of the cytomegalovirus immediate early promoter in DNA vaccines for fish. DNA fragments containing the promoter regions upstream of the rainbow trout Mx1 and interferon regulatory factor 1A (IRF1A) genes were obtained and their nucleotide sequences were determined. Vectors containing the G gene of IHNV linked to the trout promoters were found to be very effective as vaccines in rainbow trout. In addition, we demonstrated that the IRF1A promoter is transcriptionally active in mouse cells NIH-3T3 suggesting that this fish promoter might be used for mammalian DNA vaccine development as well.

摘要

构建了带有可诱导虹鳟启动子的DNA质粒载体，以取代鱼类DNA疫苗中巨细胞病毒立即早期启动子。获得了包含虹鳟Mx1和干扰素调节因子1A（IRF1A）基因上游启动子区域的DNA片段，并测定了它们的核苷酸序列。发现含有与鳟鱼启动子相连的传染性造血器官坏死病毒（IHNV）G基因的载体作为虹鳟疫苗非常有效。此外，我们证明IRF1A启动子在小鼠细胞NIH-3T3中具有转录活性，这表明该鱼类启动子也可用于哺乳动物DNA疫苗的开发。