Tamura Kazuhiro, Hara Takahiko, Yoshie Mikihiro, Irie Shinya, Sobel Andre, Kogo Hiroshi
Department of Pharmacology, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Endocrinology. 2003 Apr;144(4):1464-73. doi: 10.1210/en.2002-220834.
We used the library subtraction technique to identify genes specifically expressed in the rat uterus during early pregnancy. One such gene was that for stathmin, a factor that is associated with tubulin binding and the destabilization of microtubules. Stathmin was expressed at higher levels in implantation sites than in interimplantation sites on d 6 and 7 of pregnancy; the levels on d 6 and 7 were higher in implantation sites than in the entire uterus on d 3-5 of pregnancy or in nonpregnant uteri. Intense expression of stathmin mRNA was primarily limited to the subluminal stromal cells at the implantation site. Expression was also detected in the decidual zones and was accentuated during the period of decidualization (d 7-12). In the delayed implantation pregnant rat model, uterine stathmin expression was low, but increased after implantation induced by administration of 17beta-estradiol to the progesterone-primed animal. Further, decidualization in the pseudopregnant rat, induced by intrauterine infusion of oil, enhanced stathmin expression. Stathmin expression clearly increases in the uterus when stimulated by embryo implantation and decidualization and may play a role in the early stages of pregnancy.
我们采用文库消减技术来鉴定大鼠妊娠早期子宫中特异性表达的基因。其中一个这样的基因是与微管蛋白结合及微管去稳定化相关的一种因子——微管相关蛋白2。在妊娠第6天和第7天，微管相关蛋白2在着床部位的表达水平高于非着床部位；妊娠第6天和第7天着床部位的水平高于妊娠第3 - 5天整个子宫或未孕子宫中的水平。微管相关蛋白2 mRNA的强烈表达主要局限于着床部位的腔下基质细胞。在蜕膜区也检测到表达，并且在蜕膜化期间（第7 - 12天）表达增强。在延迟着床的妊娠大鼠模型中，子宫微管相关蛋白2的表达较低，但在给用孕酮预处理的动物注射17β - 雌二醇诱导着床后表达增加。此外，经子宫内注入油诱导的假孕大鼠的蜕膜化增强了微管相关蛋白2的表达。当受到胚胎着床和蜕膜化刺激时，子宫中微管相关蛋白2的表达明显增加，并且可能在妊娠早期发挥作用。
