Mattsson Göran, Jansson Leif, Nordin Astrid, Carlsson Per-Ola
Department of Medical Cell Biology, Biomedical Center, Uppsala University, Uppsala, Sweden.
Transplantation. 2003 Mar 15;75(5):736-9. doi: 10.1097/01.TP.0000052592.92966.FE.
Pancreatic islets are avascular immediately after transplantation and depend on revascularization. Recently, the authors found decreased vascular density in mouse islets 1 month after implantation into nondiabetic recipients. This study investigated possible differences in revascularization between islets implanted into nondiabetic and diabetic recipients, and also evaluated changes in vascular density up to 6 months posttransplantation.
Islets were syngenically transplanted beneath the renal capsule of normoglycemic or alloxan-diabetic C57BL/6 mice. One to 6 months later, the animals were killed and the grafts removed. Histologic slides were prepared and stained with Bandeiraea simplicifolia.
The vascular density in all transplanted islets was decreased compared with native islets. There were no differences in the islet graft vascular density between nondiabetic and diabetic animals. No improvement over time occurred.
The vascular density is decreased in islets implanted to cure diabetic recipients. No improvement occurs in transplanted islets after 1 month posttransplantation.
胰岛移植后即刻无血管，依赖于血管再生。最近，作者发现将胰岛植入非糖尿病受体1个月后，小鼠胰岛中的血管密度降低。本研究调查了植入非糖尿病和糖尿病受体的胰岛在血管再生方面可能存在的差异，并评估了移植后长达6个月的血管密度变化。
将胰岛同基因移植到血糖正常或用四氧嘧啶诱导糖尿病的C57BL/6小鼠的肾被膜下。1至6个月后，处死动物并取出移植物。制备组织学切片并用单叶豆凝集素染色。
与天然胰岛相比，所有移植胰岛的血管密度均降低。非糖尿病和糖尿病动物的胰岛移植血管密度无差异。随着时间推移未出现改善。
为治疗糖尿病受体而植入的胰岛血管密度降低。移植后1个月，移植胰岛未出现改善。
