Danilewicz Marian, Sikorska Beata, Wagrowska-Danilewicz Małgorzata
Department of Pathomorphology, Morphometry Division, Medical University of Łódź, Poland.
Med Sci Monit. 2003 Mar;9(3):MT42-7.
Matrix metalloproteinases (MMPs) are implicated in several pathological conditions, including cancer invasion and metastases. Among the MMPs associated with the tumor cell surface, gelatinase A (MMP2) is believed to be particularly important for cancer invasion.
MATERIAL/METHODS: The immunoexpression of matrix metalloproteinase MMP2 and its inhibitor TIMP2 was investigated in 40 laryngeal carcinomas, in order to estimate the prognostic significance of these factors. The tissue sections were studied morphometrically using computer image analysis.
The mean value of MMP2 immunoexpression in squamous cell carcinoma in patients without lymph node involvement was 7.36I7.3, and the mean value of MMP2 immunostaining in patients with lymph node metastases was 23.24I14.9. The difference between groups was statistically significant (P<0.001). Statistical analysis did not reveal a significant difference in TIMP2 expression between groups with metastases (mean: 11.38I7.7) and without (mean: 11.47I9.1). The survival time of patients with tumors with higher MMP2 expression (greater than the median, 9.6) was significantly shorter (P<0.004) than those with tumors showing low MMP2 expression (<9.6). The survival analysis revealed no significant difference between the survival time of patients in groups with high (> the median, 10.6) and low (<10.6) expression of TIMP2.
Our results suggest that MMP2 expression may be of prognostic importance in laryngeal cancer. However, imbalances in the extracellular activities of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases, linked to pathological tissue destruction, are more complex and need further investigation.
基质金属蛋白酶（MMPs）与多种病理状况有关，包括癌症侵袭和转移。在与肿瘤细胞表面相关的MMPs中，明胶酶A（MMP2）被认为对癌症侵袭尤为重要。
材料/方法：研究了40例喉癌中基质金属蛋白酶MMP2及其抑制剂TIMP2的免疫表达情况，以评估这些因素的预后意义。使用计算机图像分析对组织切片进行形态计量学研究。
无淋巴结转移患者的鳞状细胞癌中MMP2免疫表达平均值为7.36±7.3，有淋巴结转移患者的MMP2免疫染色平均值为23.24±14.9。两组间差异具有统计学意义（P<0.001）。统计分析未显示有转移组（平均值：11.38±7.7）和无转移组（平均值：11.47±9.1）之间TIMP2表达存在显著差异。MMP2表达较高（大于中位数9.6）的肿瘤患者的生存时间明显短于MMP2表达较低（<9.6）的肿瘤患者（P<0.004）。生存分析显示，TIMP2高表达（>中位数10.6）和低表达（<10.6）组患者的生存时间之间无显著差异。
我们的结果表明，MMP2表达可能对喉癌具有预后意义。然而，与病理性组织破坏相关的基质金属蛋白酶和基质金属蛋白酶组织抑制剂的细胞外活性失衡更为复杂，需要进一步研究。
