基因组转录活性与染色质结构。

Genomic transcriptional activity and the structure of chromatin.

作者信息

Reeves R, Jones A

出版信息

Nature. 1976 Apr 8;260(5551):495-500. doi: 10.1038/260495a0.

Abstract

Nucleic acid hybridisation has shown that micrococcal nuclease-derived chromatin subunits from the cells of Xenopus laevis contain fragments of ribosomal 28S, 18S and 5S RNAS within the population of 200 base-pair pieces of DNA. Subunits from cultured embryonic cells actively transcribing ribosomal RNA contain only 70-74% of the cistrons present in undigested wild-type DNA, while subunits from adult erythrocytes not active in RNA transcription contain close to 90% of the ribosomal cistrons in native chromatin.

摘要

核酸杂交分析表明，非洲爪蟾细胞内微球菌核酸酶衍生的染色质亚基，在其200个碱基对的DNA片段群体中含有核糖体28S、18S和5S RNA的片段。来自活跃转录核糖体RNA的培养胚胎细胞的亚基，仅含有未消化的野生型DNA中70 - 74%的顺反子，而来自不活跃于RNA转录的成年红细胞的亚基，含有天然染色质中近90%的核糖体顺反子。