肯尼亚西部农村地区流行的HIV变体中env CRF-10的鉴定。

Identification of env CRF-10 among HIV variants circulating in rural western Kenya.

作者信息

Songok Elijah M, Lihana Raphael W, Kiptoo Michael K, Genga Isaiah O, Kibaya Rukia, Odhiambo Frida, Kobayashi Kana, Ago Yumi, Ndembi Nicaise, Okoth Frederick, Fujiyama Yoshihide, Imanishi Jiro, Ichimura Hiroshi

机构信息

Department of Viral Infection and International Health, Graduate School of Medical Sciences, Kanazawa University, Japan.

出版信息

AIDS Res Hum Retroviruses. 2003 Feb;19(2):161-5. doi: 10.1089/088922203762688676.

DOI:10.1089/088922203762688676

PMID:12643281

Abstract

As part of a program to determine the genetic diversity of human immunodeficiency virus in rural Kenya, we carried out a molecular analysis of the C2-V3 region of HIV-infected blood samples obtained from 30 antenatal clinic attendees of seven health centers in western Kenya. Direct sequencing was carried out on the envelope C2-V3 region of proviral DNA. On phylogenetic analysis with reference strains, 20 were subtype Al, 2 were subtype D, 1 was subtype C, 1 was subtype G, 1 was CRF-10, 2A/D, 2A/C, and 2 were unclassified. The presence of CRF-10 and the great variety of subtypes and recombinants in such a limited sample size suggest that western Kenya may be a potential hotspot for HIV recombination in the country.

摘要

作为确定肯尼亚农村地区人类免疫缺陷病毒基因多样性项目的一部分，我们对从肯尼亚西部七个健康中心的30名产前门诊就诊者采集的HIV感染血样的C2-V3区域进行了分子分析。对前病毒DNA的包膜C2-V3区域进行了直接测序。与参考毒株进行系统发育分析后发现，20个样本为A1亚型，2个为D亚型，1个为C亚型，1个为G亚型，1个为CRF-10、2A/D、2A/C，2个未分类。在如此有限的样本量中存在CRF-10以及多种亚型和重组体，这表明肯尼亚西部可能是该国HIV重组的一个潜在热点地区。