Bruce C, Clegg C, Featherstone A, Smith J, Biryahawaho B, Downing R, Oram J
Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK.
AIDS Res Hum Retroviruses. 1994 Nov;10(11):1543-50. doi: 10.1089/aid.1994.10.1543.
DNA sequences encoding the C2-V3 regions or the C2-V5 regions of the surface glycoprotein gp120 of the human immunodeficiency virus type 1 (HIV-1) were amplified by the polymerase chain reaction (PCR) from peripheral blood mononuclear cells obtained in 1990/1992 from 20 infected Ugandans. The PCR-amplified DNA was cloned into a phagemid vector and between 1 and 12 clones from each provirus were sequenced. The Ugandan proviruses were aligned into four subtypes (A, B, C, and D) by phylogenetic analysis of consensus nucleotide sequences for the C2-V3 regions. Analysis of the deduced amino acid sequences of the C2-V3 regions by a maximum parsimony program gave a similar phylogenetic relationship. The data indicated that phylogenetic analysis of nucleotide and/or amino acid sequences from the C2-V3 regions is a reliable method of subtype determination. The consensus amino acid sequence of the subtype A and D proviruses were almost identical to those of the Albert et al. group B and group A proviruses, respectively. The deduced amino acid sequences of the C2-V5 regions of six of these proviruses showed considerable diversity both between patients and within patients. The region varied in length between 234 and 243 amino acids and included deletions and repetitions, particularly in the V4 region.
1990年至1992年期间，从20名受感染的乌干达人身上获取外周血单核细胞，通过聚合酶链反应（PCR）扩增编码人类免疫缺陷病毒1型（HIV-1）表面糖蛋白gp120的C2-V3区域或C2-V5区域的DNA序列。将PCR扩增的DNA克隆到噬菌粒载体中，并对每个原病毒的1至12个克隆进行测序。通过对C2-V3区域的共有核苷酸序列进行系统发育分析，将乌干达原病毒分为四个亚型（A、B、C和D）。通过最大简约法程序对C2-V3区域推导的氨基酸序列进行分析，得到了相似的系统发育关系。数据表明，对C2-V3区域的核苷酸和/或氨基酸序列进行系统发育分析是确定亚型的可靠方法。A亚型和D亚型原病毒的共有氨基酸序列分别与Albert等人的B组和A组原病毒的序列几乎相同。其中六种原病毒C2-V5区域推导的氨基酸序列在患者之间以及患者体内均表现出相当大的多样性。该区域长度在234至243个氨基酸之间变化，包括缺失和重复，特别是在V4区域。
