Maksan S M, Paulo H, Ryschich E, Kuntz C, Gebhard M M, Klar E, Schmidt J
Department of Surgery, University of Heidelberg, Heidelberg, Germany.
Dig Dis Sci. 2003 Feb;48(2):279-90. doi: 10.1023/a:1021919224972.
The rising incidence of unresectable hepatocellular malignancies remains a therapeutic challenge. Little is known about the mechanisms of angiogenesis and immunological aspects of liver tumor vessels. The aim of this study was to investigate hepatic and tumor microcirculation and leukocyte-endothelium interaction by means of intravital microscopy in a rat model of hepatocellular carcinoma. Off-line analysis showed that the angioarchitecture as well as blood flow velocity in liver cancer is heterogeneous. The leukocyte-endothelium interaction is significantly reduced compared to normal liver tissue. The data suggest that the main mechanism is a reduced expression of adhesion molecules demonstrating an effective immune escape mechanism of this tumor. The model represents a useful experimental tool to explore angiogenesis inhibition or immunological therapeutic strategies in experimental liver cancer.
不可切除肝细胞恶性肿瘤的发病率不断上升，这仍然是一个治疗挑战。关于肝肿瘤血管生成机制和免疫方面知之甚少。本研究的目的是通过活体显微镜观察，在肝细胞癌大鼠模型中研究肝脏和肿瘤的微循环以及白细胞与内皮细胞的相互作用。离线分析表明，肝癌的血管结构以及血流速度是异质性的。与正常肝组织相比，白细胞与内皮细胞的相互作用显著降低。数据表明，主要机制是黏附分子表达降低，这表明该肿瘤存在有效的免疫逃逸机制。该模型是探索实验性肝癌血管生成抑制或免疫治疗策略的有用实验工具。
