Shimoyama S, Gansauge F, Gansauge S, Widmaier U, Oohara T, Beger H G
Department of General Surgery, University of Ulm, Germany.
Pancreas. 1997 Mar;14(2):181-6. doi: 10.1097/00006676-199703000-00011.
To elucidate the role of intercellular adhesion molecules (ICAMs), which has not been well understood in pancreas, we investigated the localization and expression of ICAM-1 by immunohistochemistry and in situ hybridization (ISH) in pancreatic adenocarcinoma and in normal pancreas. The localizations of ICAM-2 and ICAM-3 were also investigated by immunohistochemistry. In normal pancreas, acinar cells, duct epithelial cells, and Langerhans islet cells failed to stain with anti-ICAM-1, anti-ICAM-2, and anti-ICAM-3 antibodies. These cells showed no expression of ICAM-1 mRNA. On the other hand, various percentages of carcinoma cells were stained with anti-ICAM-1 antibody, while no carcinoma cells were stained with anti-ICAM-2 and anti-ICAM-3 antibodies. ICAM-1 mRNA expression was also observed in carcinoma cells, and ICAM-1 mRNA expression was associated with localization of the ICAM-1 protein. These results suggest that ICAM-1 expression is up-regulated in pancreatic adenocarcinoma cells and that ICAM-1 is involved in malignant processes in pancreas.
为阐明细胞间黏附分子（ICAMs）在胰腺中尚未被充分了解的作用，我们通过免疫组织化学和原位杂交（ISH）研究了ICAM - 1在胰腺腺癌和正常胰腺中的定位及表达。同时也通过免疫组织化学研究了ICAM - 2和ICAM - 3的定位。在正常胰腺中，腺泡细胞、导管上皮细胞和胰岛细胞均未被抗ICAM - 1、抗ICAM - 2和抗ICAM - 3抗体染色。这些细胞未显示ICAM - 1 mRNA的表达。另一方面，不同比例的癌细胞被抗ICAM - 1抗体染色，而没有癌细胞被抗ICAM - 2和抗ICAM - 3抗体染色。在癌细胞中也观察到了ICAM - 1 mRNA的表达，且ICAM - 1 mRNA的表达与ICAM - 1蛋白的定位相关。这些结果表明，ICAM - 1在胰腺腺癌细胞中的表达上调，并且ICAM - 1参与胰腺的恶性进程。
