Wetterhall Magnus, Palmblad Magnus, Håkansson Per, Markides Karin E, Bergquist Jonas
Institute of Chemistry, Department of Analytical Chemistry, Uppsala University, PO Box 531, SE-751 21 Uppsala, Sweden.
J Proteome Res. 2002 Jul-Aug;1(4):361-6. doi: 10.1021/pr025526s.
Mass spectrometry has in recent years been established as the method of choice for protein identification and characterization in proteomics. Capillary electrophoresis (CE) is a fast and efficient method for the separation of peptides and proteins. The on-line combination of CE with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) has been shown to be a powerful tool in the analysis of complex mixtures of proteins. This paper presents the first results from a proteomic analysis of human cerebrospinal fluid proteins by tryptic digestion and CE-FTICR-MS, where 30 proteins could be identified on a 95% confidence level with mass measurement errors less than 5 ppm.
近年来，质谱分析法已成为蛋白质组学中蛋白质鉴定和表征的首选方法。毛细管电泳（CE）是一种快速高效的肽和蛋白质分离方法。毛细管电泳与傅里叶变换离子回旋共振（FTICR）质谱（MS）的在线联用已被证明是分析复杂蛋白质混合物的有力工具。本文介绍了通过胰蛋白酶消化和CE-FTICR-MS对人脑脊液蛋白质进行蛋白质组学分析的首批结果，其中30种蛋白质可在95%置信水平上被鉴定，质量测量误差小于5 ppm。
