Arias Juan Manuel, Murbartián Janet, Perez-Reyes Edward
Department of Pharmacology, University of Virginia, P.O. Box 800735, 1300 Jefferson Park Avenue, Charlottesville 22908-0735, VA, USA.
Biochem Biophys Res Commun. 2003 Mar 28;303(1):31-6. doi: 10.1016/s0006-291x(03)00276-6.
We describe the cloning of a cDNA from a human testis library that encodes a novel protein with similarity to one repeat of voltage-gated Ca(2+) channels (Ca(v)). Northern and dot blot analyses indicate that the novel Ca(v)-like gene is expressed predominantly in testis and at lower levels in many other tissues. Heterologous expression of the Ca(v)-like protein did not lead to the induction of any detectable ionic current and failed to modify intracellular Ca(2+) concentrations. Similar one-repeat Ca(v)-like proteins have been cloned from Bacillus, Mus, and Homo, and appear to encode ion channels involved in renal function, axis determination, and sperm motility.
我们描述了从人睾丸文库中克隆出一个cDNA，它编码一种与电压门控Ca(2+)通道（Ca(v)）的一个重复序列相似的新蛋白。Northern印迹和斑点印迹分析表明，这个新的类Ca(v)基因主要在睾丸中表达，在许多其他组织中表达水平较低。类Ca(v)蛋白的异源表达并未导致任何可检测到的离子电流的诱导，也未能改变细胞内Ca(2+)浓度。类似的具有一个重复序列的类Ca(v)蛋白已从芽孢杆菌、小鼠和人类中克隆出来，似乎编码参与肾功能、轴决定和精子运动的离子通道。
