含抗氧化剂硒的单链Fv催化抗体对表皮细胞免受中波紫外线损伤的保护作用。
Protection of epidermal cells against UVB injury by the antioxidant selenium-containing single-chain Fv catalytic antibody.
作者信息
Sun Ye, Mu Ying, Li Weijia, Lv Shaowu, Jiang Zhihua, Zhang Kun, Zheng Keyan, Lin Feng, Yan Ganglin, Luo Guimin, Liu Junqiu, Shen Jiacong
机构信息
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, 125 Jiefang Road, Changchun 130023, People's Republic of China.
出版信息
Arch Biochem Biophys. 2003 Apr 1;412(1):90-4. doi: 10.1016/s0003-9861(03)00006-7.
The antioxidant effect of selenium-containing single-chain Fv catalytic antibody (Se-scFv2F3), a new mimic of glutathione peroxidase, was confirmed using a model system in which cultured rat skin epidermal cells were injured by ultraviolet B (UVB). The cell damage was characterized in terms of lipid peroxidation of the cells, cell viability, and cell membrane integrity. The injury effects of UVB and protection effects of Se-scFv2F3 on the cells were studied using the model system. UVB can damage the cells severely. Upon precultivation of the cells with 0.4U/ml Se-scFv2F3, however, the damage was significantly reduced as shown by the increase in cell viability, the decrease in the malondialdehyde and hydrogen peroxide levels, and the normalization of lactate dehydrogenase activity. In addition, a novel finding that Se-scFv2F3 can stimulate cultured epidermal cells to proliferate under certain conditions was observed.
含硒单链Fv催化抗体（Se-scFv2F3）是一种新型谷胱甘肽过氧化物酶模拟物，其抗氧化作用通过一个模型系统得到证实，该模型系统中培养的大鼠皮肤表皮细胞受到紫外线B（UVB）损伤。细胞损伤通过细胞脂质过氧化、细胞活力和细胞膜完整性来表征。利用该模型系统研究了UVB对细胞的损伤作用以及Se-scFv2F3对细胞的保护作用。UVB可严重损伤细胞。然而，在用0.4U/ml Se-scFv2F3预培养细胞后，细胞活力增加、丙二醛和过氧化氢水平降低以及乳酸脱氢酶活性恢复正常，表明损伤显著减轻。此外，还观察到一个新发现，即Se-scFv2F3在某些条件下可刺激培养的表皮细胞增殖。
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