Zadeh Alireza Dehghani, Keller Hansuli
Institute of Pathology, University of Bern, Bern, Switzerland.
Eur J Cell Biol. 2003 Feb;82(2):93-6. doi: 10.1078/0171-9335-00295.
We have determined the temporal and spatial relationship between cell polarization and alpha-actinin localization by analysing the redistribution of alpha-actinin and F-actin in spherical PMNs developing polarity and in polarized cells reversing polarity following localized stimulation with chemotactic peptide using micropipettes. Initially spherical PMNs develop a one-sided accumulation of alpha-actinin before lamellipodia enriched in alpha-actinin are formed. In polarized cells, alpha-actinin is concentrated at the leading front. When polarity is reversed, alpha-actinin redistribution to the uropod precedes reversal of morphological polarity and formation of new lamellipodia at the uropod. Later, lamellipodia enriched in F-actin and alpha-actinin develop at the former uropod to form a new front. The data document that redistribution of alpha-actinin is a very early event in the development of polarity, which precedes formation of lamellipodia.
我们通过分析α-辅肌动蛋白和F-肌动蛋白在球形多形核白细胞（PMN）形成极性过程中以及在用趋化肽进行局部刺激后极性反转的极化细胞中重新分布情况，确定了细胞极化与α-辅肌动蛋白定位之间的时空关系。最初呈球形的PMN在富含α-辅肌动蛋白的片状伪足形成之前，α-辅肌动蛋白会在一侧积累。在极化细胞中，α-辅肌动蛋白集中在前导前端。当极性反转时，α-辅肌动蛋白向尾足的重新分布先于形态极性的反转以及尾足处新片状伪足的形成。之后，富含F-肌动蛋白和α-辅肌动蛋白的片状伪足在先前的尾足处形成，以形成新的前端。这些数据表明，α-辅肌动蛋白的重新分布是极性发展过程中非常早期的事件，发生在片状伪足形成之前。
