Au W Y, Fung A, Man C, Ma S K, Wan T S, Liang R, Kwong Y L
University Department of Medicine, Queen Mary Hospital, Hong Kong.
Br J Haematol. 2003 Mar;120(6):1062-5. doi: 10.1046/j.1365-2141.2003.04194.x.
Seventeen patients with therapy-related myelodysplastic syndrome/acute myeloid leukaemia (t-MDS/AML) were examined for aberrant p15 gene methylation by methylation-specific polymerase chain reaction. Ten patients (58%) showed p15 methylation, which was significantly related to monosomy/deletion of chromosome 7q, but not to antecedent chemotherapy, blast count, leukaemic evolution or survival. In three of six patients with marrow samples obtained prior to the diagnosis of t-MDS/AML, p15 methylation predated disease development by up to 2 years. Bone marrow transplantation led to the disappearance of p15 methylation in one patient. These results showed that p15 methylation was an early event in the evolution of some t-MDS/AML patients.
对17例治疗相关的骨髓增生异常综合征/急性髓系白血病（t-MDS/AML）患者进行甲基化特异性聚合酶链反应检测p15基因异常甲基化。10例患者（58%）显示p15甲基化，其与7号染色体长臂单体/缺失显著相关，但与前期化疗、原始细胞计数、白血病进展或生存无关。在6例t-MDS/AML诊断前获取骨髓样本的患者中，有3例p15甲基化在疾病发生前2年就已出现。1例患者接受骨髓移植后p15甲基化消失。这些结果表明，p15甲基化是部分t-MDS/AML患者疾病进展过程中的早期事件。
