Beck Felix, Chawengsaksophak Kallayanee, Luckett Jenni, Giblett Susan, Tucci Joseph, Brown Jane, Poulsom Richard, Jeffery Rosemary, Wright Nicholas A
Department of Biochemistry, University of Leicester, University Road, Leicester, LE1 7RH, UK.
Dev Biol. 2003 Mar 15;255(2):399-406. doi: 10.1016/s0012-1606(02)00096-9.
Inactivation of Cdx2 by homologous recombination results in the development of forestomach epithelium at ectopic sites in pericaecal areas of the midgut of heterozygote mice. Local factors subsequently result in the secondary induction of tissues exhibiting an orderly sequence of tissue types between the ectopic forestomach tissue and the surrounding colon. Clonal analysis of this secondarily generated tissue using Y chromosome painting in chimaeric mice indicates that once differentiated to express Cdx2, host colonic epithelium can only form small intestinal-type epithelium, while Cdx2 mutant cells give rise to a succession of gastric-type tissue but never to a small intestine morphology. Our results indicate a difference in potency between forestomach and midgut precursor endodermal cells.
通过同源重组使Cdx2失活会导致杂合子小鼠中肠盲肠周围区域异位部位出现前胃上皮发育。局部因素随后导致在异位前胃组织和周围结肠之间出现一系列有序组织类型的组织的二次诱导。在嵌合小鼠中使用Y染色体描绘对这种二次生成的组织进行克隆分析表明，一旦分化以表达Cdx2，宿主结肠上皮只能形成小肠型上皮，而Cdx2突变细胞会产生一系列胃型组织，但绝不会形成小肠形态。我们的结果表明前胃和中肠前体内胚层细胞在潜能上存在差异。
