Sasanami Tomohiro, Pan Jianzhi, Mori Makoto
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.
J Steroid Biochem Mol Biol. 2003 Jan;84(1):109-16. doi: 10.1016/s0960-0760(03)00008-6.
Avian perivitelline membrane, an oocyte extracellular matrix homologous to the zona pellucida in mammals or chorion in fish, is composed of at least two glycoproteins. Previous studies have indicated that one of the components, a glycoprotein homologous to mammalian ZPC, is produced in the granulosa cells of the developing follicles of quail ovary on stimulation with testosterone. However, little is known about the molecular biology of the other component of the avian perivitelline membrane, ZP1, and information about gene expression is particularly lacking. We have cloned the ZP1 in Japanese quail and examined its gene expression. A cDNA encoding quail ZP1 was isolated from the livers of mature females using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. It encoded a 934-amino acid protein that showed greatest homology (87.8% identity) with the chicken ZP1. RT-PCR amplification indicated that the ZP1 mRNA in the liver was restricted to mature laying females. The expression of ZP1 mRNA was stimulated by in vivo treatment with diethylstilbestrol in immature females as well as males. These results suggested that androgens and estrogens coordinately regulate the formation of quail perivitelline membrane proteins. In addition, the use of ZP1 transcriptional induction in males or immature females as a biological marker of environmental estrogens is discussed.
禽卵黄膜是一种与哺乳动物透明带或鱼类绒毛膜同源的卵母细胞细胞外基质，由至少两种糖蛋白组成。先前的研究表明，其中一种成分，一种与哺乳动物ZPC同源的糖蛋白，是鹌鹑卵巢发育卵泡的颗粒细胞在睾酮刺激下产生的。然而，对于禽卵黄膜的另一种成分ZP1的分子生物学知之甚少，尤其是缺乏关于基因表达的信息。我们已经克隆了日本鹌鹑的ZP1并检测了其基因表达。使用逆转录-聚合酶链反应（RT-PCR）和cDNA末端快速扩增技术，从成熟雌性鹌鹑的肝脏中分离出编码鹌鹑ZP1的cDNA。它编码一种934个氨基酸的蛋白质，与鸡ZP1具有最高的同源性（87.8%的同一性）。RT-PCR扩增表明，肝脏中的ZP1 mRNA仅限于成熟的产卵雌性鹌鹑。在未成熟雌性和雄性鹌鹑中，体内注射己烯雌酚可刺激ZP1 mRNA的表达。这些结果表明，雄激素和雌激素协同调节鹌鹑卵黄膜蛋白的形成。此外，还讨论了将雄性或未成熟雌性鹌鹑中ZP1转录诱导作为环境雌激素生物标志物的应用。
