Piekarska Katarzyna, Ławrynowicz-Paciorek Maja, Magdziak Agnieszka, Bareja Elzbieta, Rudecka Anna, Wyrebiak Agata, Pietrzyk Maciej, Kochman Maria
Zakład Bakteriologii PZH w Warszawie.
Med Dosw Mikrobiol. 2002;54(4):305-15.
The aim of this study was to evaluate the drug susceptibility of Enterococcus sp. strains isolated in 2000-2001, from patients of five Warsaw's hospitals (154 strains) and from fecal samples of healthy persons (33 strains). On biochemical reaction profiles species of clinical enterococci were identified as: E. faecalis--66.2%, E. faecium--29.2%, E. hirae--1.9%, E. gallinarum--1.3%, E. casseliflavus--0.6% and E. avium--0.6%. The species of enterococci from stool's samples were identified as: E. faecalis--28 strains, E. durans--2 strains and single strains: E. faecium, E. gallinarum and E. casseliflavus. Susceptibility to 20 antibiotics was tested by disc diffusion method. None of these 187 enterococcal strains was vancomycin resistant; 3 strains of E. gallinarum and 1--E. casseliflavus demonstrated intermediately susceptibility to vancomycin, but they were susceptible to teicoplanin--phenotype Van C. Among clinical strains were resistant to penicillin--33.3% of E. faecalis and 100% of E. faecium, to ampicillin--over 80% of E. faecium and 1 strain of E. faecalis. None of these strains produced beta-lactamase. High-level resistance to aminoglicoside was expressed by 48 strains (47.1%) E. faecalis and 36 (80%) E. faecium isolated from clinical specimens. Both--HLR to streptomycin and gentamycin were found in 28.3% of E. faecalis and 68.9% of E. faecium. Among 33 strains isolated from fecal samples of healthy persons--3 of E. faecalis were resistant to streptomycin and one was resistant to both gentamicin and streptomycin. In general, enterococcal strains isolated from samples of healthy persons were susceptible to the most of used antibiotics. But to rifampicin none of these strains were susceptible. There were about 40% of E. faecalis strains isolated from healthy persons, resistant to tetracyline.
本研究旨在评估2000 - 2001年从华沙五家医院的患者（154株）及健康人的粪便样本（33株）中分离出的肠球菌属菌株的药敏情况。根据生化反应谱，临床分离的肠球菌种类鉴定如下：粪肠球菌——66.2%，屎肠球菌——29.2%，海氏肠球菌——1.9%，鹑鸡肠球菌——1.3%，卡氏黄色肠球菌——0.6%，鸟肠球菌——0.6%。粪便样本中的肠球菌种类鉴定如下：粪肠球菌——28株，耐久肠球菌——2株，还有单株的屎肠球菌、鹑鸡肠球菌和卡氏黄色肠球菌。采用纸片扩散法检测了这些菌株对20种抗生素的敏感性。这187株肠球菌菌株均对万古霉素不耐药；3株鹑鸡肠球菌和1株卡氏黄色肠球菌对万古霉素呈中度敏感，但它们对替考拉宁敏感——Van C表型。临床菌株中，对青霉素耐药的有：33.3%的粪肠球菌和100%的屎肠球菌；对氨苄西林耐药的有：超过80%的屎肠球菌和1株粪肠球菌。这些菌株均不产β - 内酰胺酶。从临床标本中分离出的48株（47.1%）粪肠球菌和36株（80%）屎肠球菌对氨基糖苷类呈现高水平耐药。同时对链霉素和庆大霉素高水平耐药的粪肠球菌占28.3%，屎肠球菌占68.9%。在从健康人粪便样本中分离出的33株菌株中，3株粪肠球菌对链霉素耐药，1株对庆大霉素和链霉素均耐药。总体而言，从健康人样本中分离出的肠球菌菌株对大多数使用的抗生素敏感。但对利福平均不敏感。从健康人分离出的粪肠球菌菌株中约40%对四环素耐药。
