Shih Jin-Yuan, Lee Yuan-Chii G, Yang Shuenn-Chen, Hong Tse-Ming, Huang Chi-Ying F, Yang Pan-Chyr
Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Clin Exp Metastasis. 2003;20(1):69-76. doi: 10.1023/a:1022598604565.
Numerous genetic changes are associated with metastasis of cancer cells. Previously, we used microarray to identify that collapsin response mediator protein-1 (CRMP-1) was involved in cancer invasion and metastasis. We further characterized that CRMP-1 was a novel invasion-suppression gene. Members of the CRMP gene family are intracellular phosphoproteins involved in the mediation of semaphorin induced F-actin depolymerization and growth cone collapse. The precise mechanism by which CRMP-I inhibits invasion is not yet clear. However, CRMP-1 transfected cells had fewer filopodia and less Matrigel-invasion abilities. A low expression of CRMP-I mRNA in lung cancer tissue was significantly associated with advanced disease, lymph node metastasis, early post-operative relapse, and shorter survival. In this article, we reviewed the functions of CRMPs and semaphorins and analyzed the structure and motifs of CRMP-1 by bioinformatics. As such, we hoped to shed further light on the mechanism by which CRMP-1 suppresses the invasion of cancer cells.
众多基因变化与癌细胞转移相关。此前，我们利用微阵列鉴定出塌陷反应介导蛋白-1（CRMP-1）参与癌症侵袭和转移。我们进一步证实CRMP-1是一种新型的侵袭抑制基因。CRMP基因家族成员是细胞内磷蛋白，参与介导信号素诱导的F-肌动蛋白解聚和生长锥塌陷。CRMP-1抑制侵袭的确切机制尚不清楚。然而，转染CRMP-1的细胞丝状伪足较少且基质胶侵袭能力较低。肺癌组织中CRMP-1 mRNA的低表达与疾病进展、淋巴结转移、术后早期复发及较短生存期显著相关。在本文中，我们综述了CRMPs和信号素的功能，并通过生物信息学分析了CRMP-1的结构和基序。因此，我们希望进一步阐明CRMP-1抑制癌细胞侵袭的机制。
