De Venanzi F, Peña F, Jiménez O, De Alvarado H
Proc Soc Exp Biol Med. 1976 May;152(1):47-51. doi: 10.3181/00379727-152-39324.
Isolated perfused fed rat livers spontaneously liberated glucose and orthophosphate to the medium; 24-hr fasted rat livers did not exhibit these phenomena. In perfused fed rat livers, glucagon (2 mug) increased glucose output and promoted orthophosphate incorporation. In perfused fed rat livers, insulin (250 or 500 mU) inhibited the spontaneous liberation of glucose and orthophosphate. Comparable doses of insulin significantly reduced the glucagon (2 mug)-induced increase in glucose output from perfused fed rat liver, but did not affect orthophosphate uptake by the organ.
分离灌流的喂食大鼠肝脏会自发地向培养基中释放葡萄糖和正磷酸盐；禁食24小时的大鼠肝脏则不会出现这些现象。在灌流的喂食大鼠肝脏中，胰高血糖素（2微克）可增加葡萄糖输出并促进正磷酸盐掺入。在灌流的喂食大鼠肝脏中，胰岛素（250或500 mU）可抑制葡萄糖和正磷酸盐的自发释放。相当剂量的胰岛素可显著降低胰高血糖素（2微克）诱导的灌流喂食大鼠肝脏葡萄糖输出增加，但不影响该器官对正磷酸盐的摄取。
