Shen Changxian, Buck Andreas K, Liu Xiangwei, Winkler Michael, Reske Sven N
Department of Nuclear Medicine, University of Ulm, Robert-Koch-Strasse 8, D-89070 Ulm, Germany.
FEBS Lett. 2003 Mar 27;539(1-3):111-4. doi: 10.1016/s0014-5793(03)00209-6.
RNA interference is the process that double-stranded RNA induces the homology-dependent degradation of cognate mRNA mediated by 21-23 nucleotide short interfering RNA (siRNA). Here, we describe a simple virus vector for efficient delivery of siRNA into mammalian cells utilizing the well-defined H1-RNA promoter and conventional adenovirus. In this pilot study, p53 was targeted by this vector. Our results demonstrate efficient and specific knock-down of p53 in breast cancer MCF-7 and lung carcinoma A549 cells and indicate a prospective application of this siRNA expressing recombinant adenovirus system in functional genomics, cancer gene therapy and virus inhibition.
RNA干扰是双链RNA诱导同源依赖性的由21 - 23个核苷酸的小干扰RNA（siRNA）介导的同源mRNA降解的过程。在此，我们描述了一种利用明确的H1 - RNA启动子和传统腺病毒将siRNA高效递送至哺乳动物细胞的简单病毒载体。在这项初步研究中，该载体靶向p53。我们的结果证明了在乳腺癌MCF - 7细胞和肺癌A549细胞中p53的高效且特异性敲低，并表明这种表达siRNA的重组腺病毒系统在功能基因组学、癌症基因治疗和病毒抑制方面具有潜在应用价值。
