从包涵体中复性蛋白质的实际考量

Practical considerations in refolding proteins from inclusion bodies.

作者信息

Tsumoto Kouhei, Ejima Daisuke, Kumagai Izumi, Arakawa Tsutomu

机构信息

Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan.

出版信息

Protein Expr Purif. 2003 Mar;28(1):1-8. doi: 10.1016/s1046-5928(02)00641-1.

DOI:10.1016/s1046-5928(02)00641-1

PMID:12651100

Abstract

Refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. We will review key parameters associated with (1) conformation of the protein solubilized from inclusion bodies, (2) change in conformation and flexibility or solubility of proteins during refolding upon reduction of denaturant concentration, and (3) the effect of small molecule additives on refolding and aggregation of the proteins.

摘要

蛋白质从包涵体中复性受多种因素影响，包括变性剂对包涵体的溶解、变性剂的去除以及小分子添加剂对复性的辅助作用。我们将综述与以下方面相关的关键参数：（1）从包涵体中溶解出的蛋白质的构象；（2）在变性剂浓度降低时蛋白质复性过程中构象、柔韧性或溶解度的变化；（3）小分子添加剂对蛋白质复性和聚集的影响。