Nakano Kyoko, Tanabe Mariko, Nakagawa Shiro, Ohta Shigeo, Suzuki Shihoko, Shimura Masako, Matuda Sadayuki
Department of Biochemistry, Kagoshima Women's Junior College, 1-59-1 Murasakibaru, Kagoshima 890-8565, Japan.
DNA Seq. 2002 Dec;13(6):363-7. doi: 10.1080/1042517021000060777.
The dihydrolipoamide succinyltransferase (DLST) gene of the alpha-ketoglutarate dehydrogenase complex (alpha-KGDC) was isolated from a rat genomic DNA library and sequenced. This gene was composed of 15 exons and 14 introns like the human DLST gene. Sequence analysis of the promoter-regulatory region of the rat DLST gene-(Dlst) showed the possible presence of a CAAT box-sequence and of the sequences for an AP-2 site and three Sp1 sites, but no TATA box-sequence was evidenced. The nucleotide sequences of introns 1 and 4 of the rat Dlst were significantly homologous to those of introns 1 and 4 of the human DLST gene. The sequence analysis of the rat Dlst suggested that the exon coding for the E3- and/or E1-binding domain may have been lost from the gene during evolution in eukaryotic DLST, possibly after mitochondrial symbiosis because prokaryotic DLST possesses the E3- and/or E1-binding domain.
从大鼠基因组DNA文库中分离并测序了α-酮戊二酸脱氢酶复合体（α-KGDC）的二氢硫辛酰胺琥珀酰转移酶（DLST）基因。该基因与人类DLST基因一样，由15个外显子和14个内含子组成。对大鼠DLST基因（Dlst）启动子调控区的序列分析表明，可能存在一个CAAT盒序列以及一个AP-2位点和三个Sp1位点的序列，但未发现TATA盒序列。大鼠Dlst内含子1和4的核苷酸序列与人类DLST基因内含子1和4的核苷酸序列具有显著同源性。大鼠Dlst的序列分析表明，在真核生物DLST的进化过程中，可能在线粒体共生之后，编码E3和/或E1结合结构域的外显子可能已从该基因中丢失，因为原核生物DLST具有E3和/或E1结合结构域。
