Huang Hua-Shan, Ito Kiyoshi, Yoshimoto Tadashi
School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852, Japan.
DNA Seq. 2002 Dec;13(6):387-90. doi: 10.1080/1042517021000038745.
The gene glpK, encoding glycerol kinase of Thermus aquaticus has been identified [Biosci. Biotechnol. Biochem., 62 (1998) 2375-2381]. In the present work, the nucleotide sequence of glpFK operon and the gene glpF encoding glycerol facilitator were determined. T. aquaticus GlpF was predicted to contain 272 amino acids with six putative transmembrane segments and two half-membrane-spanning segments that contained the motif Asn-Pro-Ala, respectively. The amino acid residues involved in the discrimination of glycerol were deduced to be Trp44, Tyr182, and Arg188.
编码嗜热水栖菌甘油激酶的基因glpK已被鉴定[《生物科学、生物技术与生物化学》，62 (1998) 2375 - 2381]。在本研究中，测定了glpFK操纵子的核苷酸序列以及编码甘油转运蛋白的基因glpF。预测嗜热水栖菌GlpF含有272个氨基酸，具有6个推定的跨膜区段和2个分别包含基序Asn - Pro - Ala的半跨膜区段。推测参与甘油识别的氨基酸残基为Trp44、Tyr182和Arg188。
